外源ABA对番茄花粉成熟的细胞学影响  

Cytological Effects of Exogenous ABA on Pollen Maturation in Tomato

作　　者：李静 季淑婷 陈林凤 解思瑾 庄新研 冷平 戴圣杰 Li Jing;Ji Shuting;Chen Linfeng;Xie Sijin;Zhuang Xinyan;Leng Ping;Dai Shengjie(College of Agriculture and Forestry Science,Linyi University,Linyi,Shandong 276000,China;College of Horticulture,China Agricultural University,Beijing 100193,China)

机构地区：[1]临沂大学农林科学学院,山东临沂276000 [2]中国农业大学园艺学院,北京100193

出　　处：《中国农业文摘（农业工程）》2019年第4期65-70,共6页Agricultural Science and Engineering in China

基　　金：山东省自然科学基金(ZR2019PC016);国家级大学生创新创业训练计划(201810452007);临沂大学博士科研启动基金(LYDX2016BS095)

摘　　要：花粉的成熟对植物的繁衍,尤其对农作物的产量和品质都有决定性的影响。花粉的成熟受极其复杂的激素网络调控,其中脱落酸是其重要的激素之一,而其对花粉成熟的调控机理尚不清楚。为探究脱落酸在花粉成熟过程中的作用,本文分别利用不同浓度的NDGA溶液和不同浓度的ABA溶液处理小孢子阶段的番茄雄蕊,于处理后的第2天、4天、6天及8天取样,利用显微镜观察花粉的细胞学形态。结果显示,不同浓度的NDGA和ABA处理均对番茄花粉的成熟产生显著影响,并不同程度地导致花粉畸形。其中,不同浓度的NDGA对花粉的影响不同,但相同处理时间内NDGA的浓度越大,异常花粉的比率越大;同一浓度下,随着处理时间的延长,异常花粉的比率增大。而不同浓度ABA溶液之间对番茄花粉的影响差异不明显,随着处理时间的延长,不同浓度ABA处理均使得异常花粉的比率增大。上述结果表明,ABA在番茄花粉成熟过程中起到重要的调控作用。Pollen maturation has a decisive effect on plant reproduction,especially on crop yield and quality. Pollen maturation is regulated by a complex hormone network. Abscisic acid is one of the most important hormones. However,the regulation mechanism of abscisic acid on pollen maturation is still unclear. In order to explore the role of abscisic acid in pollen maturation,tomato stamens at microspore stage were treated with different concentrations of NDGA solution and ABA solution, respectively. Samples were taken on the 2nd,4th,6th and 8th day after treatment,and the cytological morphology of pollen was observed by microscopy. The results showed that different concentrations of NDGA and ABA had significant effects on the maturation of tomato pollen and resulted in pollen deformity in varying degrees. Among them,different concentrations of NDGA have different effects on pollen,but the higher the concentration of NDGA in the same treatment time,the higher the rate of abnormal pollen. At the same concentration,the percentage of abnormal pollen increased with the prolongation of treatment time. The effect of different concentration of ABA solution on tomato pollen was not significant. With the prolongation of treatment time,different concentration of ABA treatment increased the ratio of abnormal pollen. The results showed that ABA played an important role in the regulation of tomato pollen maturation.

关 键 词：脱落酸 去甲二氢愈创木酸 花粉成熟 细胞学 

分 类 号：S641.2[农业科学—蔬菜学]