多疣壁虎胚胎发育过程中角蛋白的表达分析  

The mRNA Expression of Keratin Genes in Embryonic Development of Gekko japonicus

作　　者：陈铭月 王飞飞 李鹏[1] 严洁[1] 周开亚[1] Chen Mingyue;Wang Feifei;Li Peng;Yan Jie;Zhou Kaiya(School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Nanjing 210023,China)

机构地区：[1]南京师范大学生命科学学院江苏省生物多样性与生物技术重点实验室

出　　处：《南京师大学报（自然科学版）》2019年第2期99-104,共6页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31672269);江苏省高校优势学科Ⅱ期项目(PAPD);江苏省高等学校自然科学研究面上项目(17KJD240001);江苏高校品牌专业建设工程一期项目-生物科学(PPZY2015B117)

摘　　要：角蛋白包括α角蛋白和β角蛋白两种类型.迄今α角蛋白被发现存在于所有的脊椎动物中,而β角蛋白仅存在于鸟类和爬行类表皮及其附属物中.本研究通过同源搜索比对鉴定了多疣壁虎(Gekko japonicus)的 1个β角蛋白基因: G.japonicus claw keratin-like(LOC107121854),暂命名为 ge-gprp-13 ,和1个α角蛋白基因: G.japonicus keratinocyte associated protein 2(KRTCAP2),暂命名为 ge-krtcap 2 .利用荧光定量PCR方法比较分析了多疣壁虎 ge-gprp-13和ge-krtcap 2 基因在其胚胎发育过程中的mRNA表达模式.研究结果表明,在多疣壁虎胚胎发育的不同时期,α角蛋白基因( ge-krtcap 2 )和β角蛋白基因(ge-gprp-13)的表达模式不同:在趾部发育的初期,α角蛋白基因先表达,而β角蛋白基因主要在趾部发育的后期进行表达,并且在趾部形成的整个过程中,β角蛋白基因的表达量远远高于α角蛋白基因的表达量.Reptile epidermis contains two types of keratins-α-keratins and β-keratins. Up to date,α-keratins are reported to be present in all vertebrates,while β-keratins are only found in birds and reptiles. In the present study,two keratin genes of Gekko japonicus were identified from the National Center of Biotechnology Information database,one β-keratin gene, G.japonicus claw keratin-like(LOC107121854)tentatively named ge-gprp-13 and one α-keratin gene, G.japonicus keratinocyte associated protein 2(KRTCAP2)tentatively named ge-krtcap 2 . The mRNA expression of keratin genes during embryonic development of the gecko was studied by using fluorescence quantitative real-time PCR. The results showed that the α-keratin gene( ge-krtcap 2 )and the β-keratin gene( ge-gprp-13 )have different expression patterns in different stages of embryonic development of the gecko. In the early stage of toe development,the α-keratin gene is expressed first,while the β-keratin gene is mainly expressed in the later stage of toe development. And during the formation of the toe,the expression level of the β-keratin gene is much higher than the α-keratin gene.

关 键 词：多疣壁虎 α角蛋白 β角蛋白 MRNA表达 

分 类 号：Q9513[生物学—动物学]