HPLC法同时测定裸花紫珠中3种苯乙醇苷类成分的含量  

Simultaneous Determination of Three Kinds of Phenylethanoid Glycosides in Callicarpa nudiflora by HPLC Method

作　　者：李秀敏 王勇[1] 张俊清[1] 赖伟勇[1] 张旭光[1] LI Xiu-min;WANG Yong;ZHANG Jun-qing;LAI Wei-yong;ZHANG Xu-guang(Hainan provincial key laboratory of R&D on tropical herbs,School of Pharmacy,Hainan Medical University,Hainan Haikou 571199,China)

机构地区：[1]海南省热带药用植物研究开发重点实验室海南医学院药学院

出　　处：《广州化工》2019年第11期104-106,共3页GuangZhou Chemical Industry

基　　金：海南省自然科学基金创新研究团队项目(编号:2018CXTD339)

摘　　要：建立了同时测定裸花紫珠药材中毛蕊花糖苷、异毛蕊花糖苷、连翘酯苷B含量的方法。采用高效液相色谱(HPLC)法。色谱柱为Luna C18(2)(150mm×4.6mm,5μm);乙腈-0.1%醋酸溶液(1585,v/v)为流动相;流速为1.0mL/min,检测波长为332nm;柱温为30℃;进样量:10μL。结果表明:毛蕊花糖苷、异毛蕊花糖苷、连翘酯苷B分别在0.1044~1.6704、0.1024~1.6384、0.0458~0.5569μg/mL范围内呈良好线性关系(r>0.9999);三者精密度、稳定性、重复性试验的RSD<2%;平均加样回收率分别为99.80%、100.83%和100.83%,RSD分别为1.53%、1.45%和1.50%(n=6)。本实验建立的分析方法简单快速、稳定可靠,适用于裸花紫珠药材中3种活性成分毛蕊花糖苷、异毛蕊花糖苷、连翘酯苷B含量的同时测定。A method was established for simultaneous determination of ascoside,isoacteoside and forsythiaside B in Callicarpa nudiflora.The HPLC method was performed on Luna C 18(2)column(150 mm×4.6 mm,5μm)with mobile phase consisted of acetonitrile-0.1%acetic acid solution at the flow rate of 1.0 mL/min.The detection wavelength was set at 332 nm.The column temperature was 30℃.The liner range were 0.0458~0.5569μg/mL for forsythiaside B(r=0.9999),0.1044~1.6704μg/mL for ascoside(r=0.9999)and 0.1024~1.6384μg/mL for Isoacteoside(r=0.9999).RSD of precision,stability and reproducibility tests were all lower than 2%.Average recoveries were 100.83%(RSD=1.50%,n=6),99.8%(RSD=1.53%,n=6)and 100.83%(RSD=1.45%,n=6),respectively.This method is suitable for simultaneous determination of ascoside,isoacteoside,forsythiaside B in Callicarpa nudiflora resulting from its simple,fast,stable and reliable characteristics.

关 键 词：裸花紫珠 毛蕊花糖苷 异毛蕊花糖苷 连翘酯苷B 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]