雪上一枝蒿配伍黄秦艽协同抗炎作用研究  

Study on Synergistic Anti-inflammatory Effect Through Different Compatibilities of Aconitum Brachypodum Diels and Verattilla Bailonii Franch

作　　者：何彩静 陈慧[1] 黄先菊[1] HE Cai-jing;CHEN Hui;HUANG Xian-ju(School of Pharmacy,South-central University For Nationalities,Wuhan Hubei 430074,China)

机构地区：[1]中南民族大学药学院

出　　处：《湖北科技学院学报（医学版）》2019年第3期195-200,共6页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：国家自然科学基金资助项目(81873090,81374064);民族医药学会项目(No 2017KYXM-Z182-82)

摘　　要：目的探讨雪上一枝蒿总生物碱(CFA)配伍黄秦艽水提物(WVBF)对抗炎药效的影响。方法采用LPS诱导RAW264.7细胞炎症模型探讨CFA与WVBF之间的协同抗炎作用,采用等效图分析法及相互作用指数法进行分析,判断CFA与WVBF协同作用的配伍最佳比例。结果结果显示CFA组、WVBF组、CFA∶WVBF=1∶2、1∶5、1∶10组,均能显著抑制LPS诱导的RAW264.7细胞NO释放量增加,但是只有CFA∶WVBF=1∶10配伍比例下,各种浓度混合物相互作用指数均小于1,表现为协同抗炎作用;CFA组、WVBF组、CFA∶WVBF=1∶10配伍组对iNOS,TNF-α,COX-2,1L-6基因表达均有显著抑制作用,配伍组对iNOS基因抑制作用明显效优于CFA组和WVBF组,而对COX-2、IL-6和TNF-α基因表达的抑制效果相对较弱。结论CFA以及CFA和WVBF的配伍可能主要是通过抑制NO的释放、iNOS基因表达来达到协同抗炎作用,且CFA∶WVBF=1∶10为最佳配伍比例。Objective To investigate the anti-inflammatory effect of total alkaloids of Aconitum brachypodum Diels(CFA) with water extract of Verattilla bailonii Franch(WVBF). Methods LPS-induced RAW 264.7 cell model was used to explore the synergistic anti-inflammatory effects of CFA and WVBF.Isobologram analytical method and interaction index for analysis were used to determine which one is the best the compatibility ratio of CFA and WVBF. Results The results indicated that CFA、WVBF and the combination of CFA and WVBF with the ratio of 1∶ 2,1∶ 5,1∶ 10 significantly inhibited LPS-induced NO production in RAW 264.7 cell model,but only interaction index in 1∶ 10 group is less than 1,showing its synergistic anti-inflammmtory effects.The results suggested that the combination of CFA and WVBF can obviously inhibit iNOS,TNF-α,COX-2 and IL-6 mRNA expression in RAW 264.7 cell model. Conclusion CFA and the compatibility of CFA and WVBF may achieve synergistic anti-inflammatory effect mainly by inhibiting NO release and iNOS gene expression,and CFA:WVBF=1∶ 10 may be the best compatibility ratio.

关 键 词：雪上一枝蒿总生物碱 黄秦艽水提物 配伍 协同抗炎 

分 类 号：R965[医药卫生—药理学]