白杨素抑制核因子κB受体活化因子配体诱导的小鼠破骨细胞生成  

Chrysin inhibits mouse osteoclastogenesis induced by receptor activator of nuclear factor kappa B ligand

作　　者：李翔翮 罗伟 胡峻贤 杨静 韩欣赟 董世武 杨先腾[5] 李森磊[5] 鄢志辉[5] 聂瑛洁[6] 田晓宾 孙立[5] Li Xianghe;Luo Wei;Hu Junxian;Yang Jing;Han Xinyun;Dong Shiwu;Yang Xianteng;Li Senlei;Yan Zhihui;Nie Yingjie;Tian Xiaobin;Sun Li(Guizhou Medical University, Guiyang 550025, Guizhou Province,China;Department of Orthopedics, the First Hospital Affiliated to Army Medical University, Chongqing 400038,China;Department of Traumatic Orthopedics, Daping Hospital, Chongqing 400042, China;Department of Biomedical Materials, Army Military Medical University, Chongqing 400038, China;Department of Orthopedics,Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China;Central Laboratory, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China)

机构地区：[1]贵州医科大学,贵州省贵阳市550025 [2]陆军军医大学第一附属医院骨科,重庆市400038 [3]大坪医院创伤外科,重庆市400042 [4]陆军军医大学生物医学材料学教研室,重庆市400038 [5]贵州省人民医院骨科,贵州省贵阳市550002 [6]贵州省人民医院中心试验室,贵州省贵阳市550002

出　　处：《中国组织工程研究》2019年第25期3978-3986,共9页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81560356),项目负责人:田晓滨;贵州省社发攻关项目[(2015)3044],项目负责人:孙立~~

摘　　要：背景:白杨素存在于多种天然植物提取物的黄酮醇类化合物,具有广泛的治疗作用并且参与机体内的炎症反应,而炎症反应可增强破骨细胞生成从而导致骨侵蚀。目的:探究白杨素在炎症环境和非炎症环境下对破骨细胞分化的影响以及对骨侵蚀的保护作用。方法:选择RAW264.7细胞为种子细胞,首先,通过核因子κB受体活化因子配体(50μg/L)、巨噬细胞集落刺激因子(25μg/L)将细胞诱导分化为破骨细胞后,以0,20,40,60μg/L白杨素干预。其次,通过脂多糖模拟炎症环境,并将RAW264.7细胞在脂多糖诱导的炎症环境下诱导分化为破骨细胞,观察0,20,40,60μg/L白杨素在炎症环境下对破骨细胞分化的影响。结果与结论:(1)白杨素有效抑制破骨细胞分化,在60μg/L时抑制效果达到最大;(2)白杨素显著抑制了破骨细胞的骨吸收功能,提示白杨素对破骨细胞造成的骨侵蚀具有保护作用;(3)白杨素通过核因子κB信号通路,抑制多种破骨细胞分化关键蛋白和基因表达;(4)白杨素对炎症有明显的抑制作用,并对炎症环境导致的破骨细胞分化有强烈的抑制作用。BACKGROUND: Chrysin(5,7-dihydroxyflavone) is a flavonol in many natural plant extracts. It has a wide range of therapeutic effects and is involved in inflammatory reactions in the body that can enhance osteoclast formation and lead to bone erosion.OBJECTIVE: To investigate the effects of chrysin on osteoclast differentiation and its protective effect on bone erosion in inflammatory and non-inflammatory environments.METHODS: RAW264.7 cells were selected as seed cells. First, the RAW264.7 cells were induced with receptor activator of nuclear factor kappa B ligand(50 μg/L) and macrophage colony-stimulating factor(25 μg/L) to generate osteoclasts. The cells were randomly divided into four groups according to chrysin concentration(0, 20, 40, and 60 μg/L). Second, lipopolysaccharide was used to simulate the inflammatory environment. RAW264.7 cells were induced by lipopolysaccharide to differentiate into osteoclasts, and the effect of different concentrations of chrysin(0, 20, 40, 60 μg/L) on osteoclast differentiation was observed in the same way.RESULTS AND CONCLUSION: Chrysin effectively inhibited osteoclast differentiation, with the maximum effect at 60 μg/L. Chrysin significantly inhibited the bone absorption function of osteoclasts, suggesting that chrysin has a protective effect on bone erosion caused by osteoclasts. Chrysin suppressed the protein and gene expression related to osteoclast differentiation by nuclear factor kappa B signaling pathway. Therefore, chrysin has an anti-inflammatory effect and it is also powerful to inhibit osteoclast differentiation in an inflammatory environment.

关 键 词：白杨素 破骨细胞生成 炎症性骨破坏 炎症反应 脂多糖 骨侵蚀 核因子ΚB信号通路 核因子ΚB受体活化因子配体 

分 类 号：R453[医药卫生—治疗学] R392[医药卫生—临床医学]