外源性神经生长因子和碱性成纤维细胞生长因子联合应用促进重型脑创伤模型大鼠内源性脑细胞的增殖  

Combined use of exogenous nerve growth factor and basic fibroblast growth factor promotes proliferation of endogenous brain cells in a rat model of severe traumatic brain injury

作　　者：王童[1] 刘阳[1] 朱业淘 Wang Tong;Liu Yang;Zhu Yetao(Department of Neurosurgery, the Third Hospital of Mianyang/Sichuan Mental Health Center, Mianyang621000, Sichuan Province, China)

机构地区：[1]绵阳市第三人民医院(四川省精神卫生中心)神经外科

出　　处：《中国组织工程研究》2019年第25期3998-4003,共6页Chinese Journal of Tissue Engineering Research

基　　金：四川省卫生和计划生育委员会支持项目(17PJ183),项目负责人:刘阳;四川省医学会支持项目(S16038),项目负责人:刘阳;绵阳市卫生和计划生育委员会支持项目(201615),项目负责人:刘阳~~

摘　　要：背景:研究证明神经生长因子、碱性成纤维细胞生长因子能够明显在体外促进神经干细胞的自我更新及分化,二者联合应用对成年大鼠脑创伤后内源性脑细胞的影响研究甚少。目的:探索外源性神经生长因子和碱性成纤维细胞生长因子对重型脑创伤大鼠内源性大脑组织细胞的影响。方法:以改良Feeney氏法对48只SD大鼠进行脑创伤造模,造模后随机将48只大鼠分为神经生长因子组、碱性成纤维细胞生长因子组、联合组、对照组。造模后24 h,各组分别在脑室内注入对应的神经营养因子,对照组注射生理盐水。采用行为学实验观察肢体恢复情况,免疫组化法比较各组大鼠脑内BrdU阳性细胞的表达。结果与结论:①从造模第5天开始神经生长因子组、碱性成纤维细胞生长因子组、联合组行为学实验评分均小于对照组(P <0.05),且联合组评分低于神经生长因子组、碱性成纤维细胞生长因子组(P <0.05);②神经生长因子组、碱性成纤维细胞生长因子组、联合组BrdU阳性细胞数量明显多于对照组,差异有显著性意义(P <0.05),同时联合组BrdU阳性细胞明显多于神经生长因子组、碱性成纤维细胞生长因子组(P <0.05);③结果表明,外源性神经生长因子和碱性成纤维细胞生长因子可加快脑创伤大鼠肢体功能恢复和促进大脑组织细胞的增殖,且神经生长因子和碱性成纤维细胞生长因子的联合使用能取得更加显著的效果。BACKGROUND: Either nerve growth factor or basic fibroblast growth factor can promote the self-renewal and differentiation of neural stem cells in vitro. Little has been reported on the effect of the combination of these two factors on endogenous brain cells after traumatic brain injury in adult rats.OBJECTIVE: To explore the effects of exogenous nerve growth factor and basic fibroblast growth factor on the proliferation of endogenous brain cells in the rats with severe traumatic brain injury.METHODS: Animal models of traumatic brain injury were made in 48 adult Sprague-Dawley rats using modified Feeney’s method, and then randomized into 4 groups: nerve growth factor, basic fibroblast growth factor, combination(injection of nerve growth factor and basic fibroblast growth factor), and control(normal saline injection) groups. Intraventricular injection of different factors was performed correspondingly at 24 hours after modeling. The restoration of limb function was assessed through behavior observation. BrdU-positive cells in the brain were counted and compared among four groups using immunohistochemical method.RESULTS AND CONCLUSION:(1) Initially from the 5 th day after modeling, the behavioral test scores in the three treatment groups were significantly lower than those in the control group(P < 0.05), and the behavioral test scores in the combination group were significantly lower than those in the nerve growth factor and basic fibroblast growth factor groups(P < 0.05).(2) There were more BrdU-labeled cells in the three treatment groups than the control group(P < 0.05). The number of BrdU-positive cells in the combination group was significantly higher than that in the nerve growth factor and basic fibroblast growth factor groups(P < 0.05). In summary, the proliferation of brain cells and restoration of limb function after traumatic brain injury can be promoted by nerve growth factor or basic fibroblast growth factor. Morever, this effect can be considerably improved by the combination of nerve growth factor an

关 键 词：脑创伤 神经干细胞 神经生长因子 碱性成纤维细胞生长因子 BrdU阳性细胞 行为学实验 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]