出芽短梗霉新菌株RM1603产普鲁兰多糖条件优化及多糖分析  

Optimum Conditions and Identification of Pullulan Produced by a New Finding Strain Aureobasidium pullulans RM1603

作　　者：沈琦 张殿鹏[2] 郝雅荞 罗翔莲 杨佳瑶 魏步云 李欧[1] 赵洪新[1] SHEN Qi;ZHANG Dian-Peng;HAO Ya-Qiao;LUO Xiang-Lian;YANG Jia-Yao;WEI Bu-Yun;LI Ou;ZHAO Hong-Xin(Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018;Institute of Plant Protection and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097;College of Life Science, Shenyang Normal University, Shenyang 110034, China)

机构地区：[1]浙江理工大学生命科学学院,浙江省植物与次生代谢重点实验室,浙江杭州310018 [2]北京市农林科学院植物保护环境保护研究所,北京100097 [3]沈阳师范大学生命科学学院,辽宁沈阳110034

出　　处：《生物技术通讯》2019年第3期385-390,共6页Letters in Biotechnology

基　　金：国家农业部“减肥减药”作物免疫调控与物理防控技术及产品研发项目(2017YFD0200908);浙江省科技厅公益技术应用研究计划(2017C32050)

摘　　要：目的:出芽短梗霉RM1603是一株高产胞外多糖新菌株,通过优化其产糖条件,鉴定其多糖结构,为进一步开发利用RM1603产胞外多糖奠定理论基础。方法:以RM1603为出发菌株,在单因素分析确定最佳氮源与无机盐的基础上,利用正交试验探究RM1603最佳发酵产糖条件;薄层层析及红外光谱分析确定胞外多糖产物结构。结果:出芽短梗霉菌RM1603的初始多糖产量为28.91g/L,发酵条件优化后产糖量达到65.213g/L,提高了约2.3倍;结构分析表明RM1603的多糖产物为普鲁兰多糖。结论:出芽短梗霉RM1603是一株具有较大产糖优势,极具开发潜力的高产普鲁兰糖新菌株;奠定了开发利用RM1603生产普鲁兰多糖的理论基础。Objective: To optimize the polysaccharide production conditions for a new finding strain of high- yield extracellular polysaccharide Aureobasidium pullulans RM1603, and to identify the product. Methods: The optimal nitrogen source and inorganic salt for strain A.pullulans RM1603 cultivation were determined by monofactor analysis. The orthogonal fermentation test was used to explore the optimal fermentation conditions of RM1603. The structure of the extracellular polysaccharide was determined by thin layer chromatography and infrared spectrum analysis. Results: The polysaccharide yield of A.pullulans RM1603 was initial 32.91 g/L and optimal 65.213 g/L,which increased by 2.3 times after optimization of fermentation conditions. Structural analysis showed that the polysaccharide product of RM1603 was pullulan. Conclusion: This study demonstrates that A.pullulans RM1603 is a potential strain for high-yield pullulan, and lays the theoretical foundation for the exploitation and utilization of pullulan produced by RM1603.

关 键 词：出芽短梗霉菌 普鲁兰多糖 正交试验 薄层层析 红外光谱 

分 类 号：Q935[生物学—微生物学]