地高辛诱发小鼠消化性溃疡的实验研究  

Digoxin Induces Peptic Ulcer in Mice

作　　者：范楠[1] 夏恒 李月[1] 殷华[1] 段为钢[1] FAN Nan;XIA Heng;LI Yue;YIN Hua;DUAN Weigang(Key Laboratory of Molecular Biology for Sinomedicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China)

机构地区：[1]云南中医学院中医药学分子生物学重点实验室

出　　处：《云南中医学院学报》2018年第5期6-10,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：云南省中青年学术技术带头人后备人(2010CI043);云南省大学生创新创业计划(2018003)

摘　　要：目的证实地高辛是否具有诱发消化性溃疡的作用。方法用25%乙醇生理盐水溶解地高辛,取80只昆明种雌鼠随机分为对照组(溶剂对照)和地高辛低(10mg/kg)、中(20mg/kg)、高(40mg/kg)剂量组,每只于背部皮下注射相应剂量的药物。另取60只昆明种雌鼠,随机分为对照组(生理盐水)和地高辛口服溶液低(10mg/kg)、高(20mg/kg)剂量组,自由饮用给药。连续给药5天,每天记录鼠重变化,5天后处死动物,收集胃液和十二指肠液并测pH,观察胃窦和十二指肠形态变化,用4%甲醛溶液固定组织后进行常规石蜡切片和HE染色检测。结果小鼠注射地高辛后,体重增长缓慢,中、高剂量组胃液pH明显下降,胃、十二指肠充血明显;口服给药后,小鼠体重明显减轻,低剂量组小鼠胃液pH明显下降,胃、十二指肠明显充血,且十二指肠出血明显。结论地高辛能诱发消化性溃疡且口服给药比注射严重。Objective The main aim of the study was to verify that digoxin was able to cause peptic ulcer. Methods Digoxin injection was prepared by dissolving digoxin powder in normal saline for injection containing 25% alcohol. Kunming mice were subcutaneously injected with normal saline for injection containing 25% alcohol, or with digoxin of 10, 20 and 40 mg/kg, respectively. In an oral administration experiment, mice were administrated with normal saline, or with oral digoxin solution of 10 and 20 mg/kg via free drink, respectively. Mice were treated for 5 days, and their body weight was recorded. In the end of the experiment, animals were killed, and acidity of the stomach and duodenum juice was detected. The general morphology of gastric antrum and duodenum was observed, and then, the tissues were fixed in 4% methanal, embedded in paraffin, and stained with hematoxylin-eosin. Results Mice injected with digoxin grew more slowly than those in control group, pH of stomach juice in mice treated with digoxin of 10 and 20 mg/kg significantly decreased, and mucosa of gastric antrum and duodenum congested. In the oral experiment, when mice administrated with digoxin solution, their body weight decreased, pH of stomach juice in the mice treated with digoxin of 10 mg/kg significantly decreased, mucosa of gastric antrum and duodenum also congested, and especially, mucosa of duodenum bled obviously. Conclusion Digoxin was able to induce peptic ulcer, and the effect of the drug administrated through oral was more powerful than that through injection.

关 键 词：地高辛 消化性溃疡 小鼠 十二指肠出血 

分 类 号：R96[医药卫生—药理学] R573.1[医药卫生—药学]