梅花PmERF4基因的克隆及其表达分析  

Cloning and expression of PmERF4 gene in Prunus mume

作　　者：彭婷 冯蓝萍 王艺琴 任静[1] 包满珠[2] 张俊卫[2] PENG Ting;FENG Lanping;WANG Yiqin;REN Jing;BAO Manzhu;ZHANG Junwei(Key Laboratory of Germplasm Innovation on Protection and Conservation ofMountain Plant Resources,Ministry of Education/College of Agriculture,Guizhou University,Guiyang 550025,China;Key Laboratory of Horticultural Plant Biology,Ministry of Education/College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]贵州大学农学院/山地植物资源保护与种质创新教育部重点实验室,贵阳550025 [2]华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉430070

出　　处：《华中农业大学学报》2019年第4期20-26,共7页Journal of Huazhong Agricultural University

基　　金：贵州省科技支撑计划([2018]2302);贵州省教育厅青年科技人才成长项目([2017]112)

摘　　要：为阐明梅花(Prunusmume)ERF基因的功能,采用RT-PCR技术从梅花品种‘雪梅’中克隆获得1个PmERF4基因。生物信息学分析表明该基因cDNA全长序列为842bp,完整开放阅读框(ORF)为690bp,编码229个氨基酸。氨基酸序列比对发现,PmERF4蛋白包含1个AP2/ERF保守结构域。系统进化分析结果显示,梅花PmERF4蛋白与李亚科李属植物的ERF蛋白高度同源。利用荧光定量PCR(qRT-PCR)检测PmERF4基因在非生物胁迫和激素处理下的表达,结果表明:PmERF4基因受低温、氧化胁迫的诱导,但响应程度不同,它能够持续且强烈地响应低温胁迫,对甘露醇、MeJA、SA处理不敏感,而高盐和ABA则抑制其表达;PmERF4基因可能在梅花低温胁迫应答中发挥重要作用。ERF (ethylene response factor) transcription factor plays a critical role in response to environmental stress and hormonal signal transduction in plants.To elucidate the function of ERF gene in Prunus mume ,the PmERF4 gene was cloned from a Prunus mume ‘Xuemei’ by RT-PCR.The full-length cDNA sequence of PmERF4 was 842 bp with an open reading frame (ORF) of 690 bp,encoding 229 amino acids.The deduced amino acid sequence alignment of PmERF4 contained a conserved AP2/ERF domain.The result of phylogenetic analysis showed the PmERF4 was highly homologous to other proteins in Prunus genus.qRT-PCR was conducted to examine the expression patterns of PmERF4 under abiotic stress and hormone treatment.The results showed that PmERF4 was induced by cold and H 2 O 2 stress.The induction levels of the gene were different between cold and H 2 O 2 stress.A continuous and strong response of PmERF4 was triggered by cold. PmERF4 was insensitive to mannitol,MeJA and SA treatment and its expression was inhibited by NaCl and ABA.It is indicated that PmERF4 from Prunus mume may be involved in response to cold and H 2 O 2 stress.

关 键 词：梅花 ERF转录因子 非生物胁迫 激素 基因克隆 基因表达 

分 类 号：S685.17[农业科学—观赏园艺]