丙泊酚成瘾大鼠的心理生理学特性  

Psychophysiological observation of propofol addiction in rats

作　　者：徐文婷[1,2] 杨丽勤 张忠楠 梁俊 江楠 蒋淼 郭文俊 汪萌芽[1] XU Wenting;YANG Liqin;ZHANG Zhongnan;LIANG Jun;JIANG Nan;JIANG Miao;GUO Wenjun;WANG Mengya(Cell Electrophysiology Laboratory,Wannan Medical College,Wuhu 241002,Anhui,China;Rising-Star Group,Wannan Medical College,Wuhu 241002,Anhui,China;School of Anesthesiology,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院细胞电生理研究室,,安徽芜湖241002 [2]皖南医学院启明星小组,安徽芜湖241002 [3]皖南医学院麻醉学院,安徽芜湖241002

出　　处：《中国临床药理学与治疗学》2019年第6期608-614,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家级大学生创新创业训练计划项目(201710368017);国家自然科学基金项目(31271155)

摘　　要：目的:探讨大鼠对丙泊酚的成瘾性及其心理生理学特征。方法:选用成年雄性SD大鼠33只,随机分为丙泊酚组(Propofol Group,n=18)和对照组(Control Group,n=15),在一周适应性饲养后,接受为期11天的条件性位置偏爱(CPP)实验,包括前测阶段、训练阶段和后测阶段。CPP实验后,在乌拉坦麻醉下分别对大鼠伏隔核核心部(Acbc)和基底外侧杏仁核(BLA)生物电活动进行细胞外记录,并同步记录体温、心电图(ECG)、呼吸肌肌电(EMG),观察腹腔注射30mg/kg丙泊酚的影响。结果:(1)丙泊酚组大鼠后测阶段在伴药箱停留时间较前测阶段增加(P<0.01),丙泊酚组大鼠后测阶段与前测阶段在伴药箱停留时间的差值较对照组增加(P<0.05);(2)丙泊酚组(n=10)大鼠Acbc和BLA的放电频率较对照组(n=5)均升高(Acbc:P<0.01,BLA:P<0.05);(3)丙泊酚组大鼠核团放电频率的Acbc/BLA比值高于对照组大鼠(P<0.05);(4)急性腹腔注射30mg/kg丙泊酚后,两核团放电频率均无明显变化,但丙泊酚组大鼠呼吸频率、体温下降(P<0.05,n=8)。结论:短期重复给予小剂量丙泊酚即有成瘾性,其机制可能与脑内奖赏-惩罚系统的平衡改变有关。AIM : To observe the addiction action of propofol on rats and explore the possible psychophysiological characteristics of the model rats. METHODS : Thirty-three adult male SD rats were divided into two groups randomly, i.e. Propofol Group ( n =18) and Control Group ( n =15). After a week of adaptive feeding, an 11-day conditioned place preference (CPP) experiment, including pretest stage, training stage and posttest stage was conducted in all rats.After CCP experiment, the electric activities from addiction-related nuclei accumbens nucleus core (Acbc) and basolateral amygdala (BLA) were extracellularly recorded, and physiological functions of electrocardiogram (ECG), electromyogram (EMG) of respiratory muscles and body temperature were simultaneously observed. The changes of electrical activities in Acbc and BLA, and physiological functions were observed after intraperitoneal injection of NS and propofol. RESULTS :(1) There was an increase of the time in the drug box for the propofol group rats ( P < 0.01 ), and an increased difference between posttest and pretest in comparison with control group rats ( P < 0.05 ).(2) The firing rate in Acbc and BLA in propofol group rats ( n =10) was significantly higher than that in the control group rats ( n =5)(Acbc: P < 0.01 , BLA: P < 0.05 ).(3) An increased Acbc/BLA ratio of firing rate in propofol group rats was seen in comparison with control group rats ( P < 0.05 ).(4) No significant changes of firing rate were observed after injecting propofol but the respiratory rate and body temperature in propofol group rats decreased significantly ( P < 0.05 , n =8). CONCLUSION : These results indicate that the short-term repeated administration of small dose propofol may lead to addiction, which is possibly related to the changes in reward-punishment system balance in the brain of rats.

关 键 词：丙泊酚 成瘾 条件性位置偏爱 心理生理学 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R614[医药卫生—基础医学]