动态轴向压缩柱精制博来霉素  

Refining Process of Bleomycin by Dynamic Axial Compression Column

作　　者：李敏[1] 谢新宇[1] 王晶珂[1] 彭亮[1] 李梅[1] LI Min;XIE Xinyu;WANG Jingke;PENG Liang;LI Mei(NCPC New Drug Research & Development Co.,Ltd.,National Engineering ResearchCenter of Microbial Medicine,Hebei Industry Microbial Metabolic Engineering & TechnologyResearch Center,Shijiazhuang 050015,China)

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心

出　　处：《化学与生物工程》2019年第6期53-56,共4页Chemistry & Bioengineering

摘　　要：以博来霉素铜盐粗粉为原料,以高纯度A 2和B 2(A 2+B 2>90%)的收率为评价指标,开发了一种利用动态轴向压缩(DAC)柱分离纯化、脱铜为一体的博来霉素精制方法。以C18ME为层析介质,以甲醇-1.5 g·L ^-1 EDTA-2Na(18∶82,体积比)为洗脱剂,在洗脱流速为150~200 mL·min ^-1 、上样量为1.5~2.0 g·L ^-1 的最佳工艺条件下,采用DAC-HB100柱制备得到的博来霉素精粉纯度和铜盐含量均符合USP标准要求,总收率大于70%。Using crude powder of Bleomycin copper salt as material,and using the yield of Bleomycin A 2 and B 2 with high purity(A 2+B 2>90%) as an evaluation index,we developed a refining process for separation,purification,and copper removal of Bleomycin by dynamic axial compression(DAC) column.Using the C18ME silica gel as the optimal chromatographic media,and methanol-1.5 g·L ^-1 EDTA-2Na(18∶82,volume ratio) as an eluent,we prepared the pure powder of Bleomycin with the total yield more than 70% by DAC-HB100 column under the optimal sampling conditions as follows:the elution flow rate of 150-200 mL·min^-1 ,and the sampling amount of 1.5-2.0 g·L ^-1 .The purity and copper salt content of the prepared Bleomycin meet the requirements of USP standard.

关 键 词：博来霉素 分离纯化 动态轴向压缩柱 C18ME层析介质 

分 类 号：TQ460.64[化学工程—制药化工] R978.1[医药卫生—药品]