和厚朴酚对人结肠癌细胞及Wnt信号通路的影响及调控作用  

Effect of honokiol on human colon cancer cells and Wnt signaling pathway

作　　者：吴柯[1] 薛莱 韩萍 WU Ke;XUE Lai;HAN Ping(Teaching and Research Section of Pharmacology of Chongqing Medical University/Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology,Chongqing 400016,China;Department of Clinical Pharmacy,Jiangyou People′s Hospital,Jiangyou,Sichuan 621700,China;Department of Oncology,Chongqing General Hospital,Chongqing 400013,China)

机构地区：[1]重庆医科大学药理学教研室/重庆市生物化学与分子药理学重点实验室,重庆400016 [2]四川省江油市人民医院临床药学科,621700 [3]重庆市人民医院肿瘤科,400013

出　　处：《检验医学与临床》2019年第12期1643-1645,1649,共4页Laboratory Medicine and Clinic

基　　金：重庆市渝中区科技计划项目(20150134);重庆市基础与前沿计划项目(cstc2015jcyA10046)

摘　　要：目的 探讨和厚朴酚(HNK)对人结肠癌细胞及Wnt信号通路的影响和调控作用。方法 采用结晶紫染色法和增殖细胞核抗原(PCNA)蛋白表达检测HNK对人结肠癌LoVo细胞增殖的抑制作用;利用流式细胞仪和Caspase-3蛋白表达观察HNK对人结肠癌LoVo细胞凋亡的影响;利用荧光素酶报告质粒检测HNK对Wnt信号通路中β-catenin/Tcf4转录活性的影响;蛋白的表达采用Western blot检测。结果 HNK剂量依赖性地抑制细胞增殖( P <0.05),并诱导细胞凋亡。荧光素酶报告质粒检测结果显示,HNK在10.0 μmol/L时就能明显降低LoVo细胞中β-catenin/Tcf4转录活性( P <0.05)。结论 HNK能抑制人结肠癌细胞增殖,诱导其凋亡,该作用可能与HNK抑制Wnt信号通路的转导有关。Objective To investigate the anti-cancer effect of honokiol on colon cancer cells and Wnt signaling pathway. Methods The assay of crystal violet staining and the protein expression of proliferating cell nuclear antigen (PCNA) were applied to detect the proliferation effect of LoVo cells.Flow cytometric and the protein expression of Capase-3 were introduced to analyze the effect of apoptosis in LoVo cells.Luciferase reporter assay was used to measure the transcriptional activity of β-catenin/Tcf4 in LoVo cells.The protein expression was detected by Western blot. Results Honokiol inhibited the proliferation and induced the apoptosis in LoVo cells in a dose-dependent manner ( P <0.05).The transcriptional activity of β-catenin/Tcf4 in LoVo cells was inhibited by honokiol at 10.0 μmol/L ( P <0.05). Conclusion Honokiol could inhibit the proliferation and induce the apoptosis of colon cancer cells,which maybe related to the inhibition of Wnt signal transduction.

关 键 词：和厚朴酚 结肠癌细胞 增殖 凋亡 

分 类 号：R965[医药卫生—药理学]