持续压力负荷离体兔脊柱运动节段软骨终板的应力变化  

Stress changes of cartilage endplate in isolated rabbit spinal motion segment under continuous pressure load

作　　者：韩涛[1,2] 展嘉文 朱立国[1,2] 冯敏山 尹逊路[1,2] Han Tao;Zhan Jiawen;Zhu Liguo;Feng Minshan;Yin Xunlu(Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China;Beijing Key Laboratory of Chinese Manipulative Technique, Beijing 100700, China)

机构地区：[1]中国中医科学院望京医院,北京市100102 [2]中医正骨技术北京市重点实验室,北京市100700

出　　处：《中国组织工程研究》2019年第27期4286-4290,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81804120),项目负责人:展嘉文;国家自然科学基金项目(81774330),项目负责人:冯敏山;国家中医药管理局国家中医临床研究基地业务建设科研专项课题(JDZX2015274),项目负责人:朱立国;国家体育总局中医特色技术在体育运动中的应用(HXKT2017001),项目负责人:朱立国;北京地区中医骨科康复服务能力与技术平台规范化建设项目(110019),项目负责人:朱立国;中国中医科学院望京医院院级科研课题(WJYY2018-07),项目负责人:展嘉文~~

摘　　要：背景:研究表明软骨组织的改变将会影响椎间盘的功能。目的:观察持续压力负荷对离体兔脊柱运动节段软骨终板的影响。方法:将16只新西兰白兔处死后在无菌条件下取出脊柱运动节段,随机分为对照组(无压力)与压力组(持续29.4N压力),利用离体加载和培养装置进行离体培养,于培养前及培养后第3,7,14天,取2组兔终板软骨组织各10个样本,苏木精-伊红染色进行组织学观察,免疫组织化学检测基质金属蛋白酶13、Ⅱ型胶原、蛋白多糖表达。结果与结论:①压力组培养至14d时椎间盘终板软骨组织中基质染色不均匀,数量减少,软骨组织有退变趋势,与对照组相比有明显改变;②培养14 d,与培养前相比压力组增强了终板内基质金属蛋白酶13蛋白的表达,基质金属蛋白酶13蛋白平均吸光度值显著高于对照组(P <0.05);③培养3 d,压力组Ⅱ型胶原平均吸光度值显著高于对照组(P<0.05);培养7,14d,压力组Ⅱ型胶原染色较培养前强度明显降低,但与对照组相比无显著差异;④培养3,7,14 d,2组蛋白多糖染色变浅(P <0.05);培养7 d压力组蛋白多糖平均吸光度值显著低于对照组(P <0.05);⑤结果说明,持续压力会导致离体培养椎间盘模型终板退变,这一结果会为椎间盘退变过程中软骨损伤及早期防治提供有意义的数据。BACKGROUND: Changes of cartilage tissues have been shown to affect the function of intervertebral disc.OBJECTIVE: To observe the effect of continuous pressure load on the cartilage endplate of isolated rabbit spinal segment.METHODS: After the 16 New Zealand white rabbits were killed, the spinal movement segments were removed under the aseptic condition and randomly divided into control group(no pressure) and pressure group(continuous 29.4 N of pressure). The isolated culture was used in vitro and cultured in vitro. Before and after cultured for 3, 7 and 14 days, the 10 cartilage endplates in each group were removed for hematoxylin-eosin staining and expression of matrix metalloproteinase 13, collagen type II and proteoglycan were detected by immunohistochemistry, respectively.RESULTS AND CONCLUSION:(1) After 14 days of culture, the cartilage structure of the intervertebral disc endplate was almost intact, the number was reduced and tended to degenerate, which showed no significant changes compared with the control group.(2) The expression of matrix metalloproteinase 13 at 14 days after culture in the pressure group was increased compared with the baseline. The average absorbance value of matrix metalloproteinase 13 in the pressure group was significantly higher than that in the control group(P < 0.05).(3)The average absorbance value of collagen type II in the pressure group was significantly higher than that in the control group at 3 days after culture(P < 0.05). The expression of collage type II after 1 and 7 days of culture in the pressure group was significantly decreased compared with the baseline, which showed no significant difference from the control group.(4) The proteoglycan staining became slight in both groups at3, 7 and 14 days after culture(P < 0.05). The average absorbance value of proteoglycan in the pressure group was significantly higher than that in the control group at 7 days(P < 0.05).(5) In summary, continuous pressure will lead to degeneration of endplate in vitro culture, which provides evi

关 键 词：脊柱 椎间盘 软骨 椎间盘模型 运动终板 软骨终板 压力负荷 应力变化 

分 类 号：R466[医药卫生—临床医学] R318