中药石香膏促进软组织缺损模型兔修复的机制  

Mechanism of traditional Chinese medicine Shixiang plaster in promoting the repair of soft tissue defects

作　　者：朱旭[1] 沈骏[1] 秧荣昆[1] 王勇[1] 刘洋[1] 孙权[1] 贺祝英[1] 陈久毅[1] Zhu Xu;Shen Jun;Yang Rongkun;Wang Yong;Liu Yang;Sun Quan;He Zhuying;Chen Jiuyi(Guiyang University of Traditional Chinese Medicine, Guiyang 550001, Guizhou Province, China)

机构地区：[1]贵阳中医学院

出　　处：《中国组织工程研究》2019年第27期4350-4355,共6页Chinese Journal of Tissue Engineering Research

基　　金：贵州省科学技术基金(合同编号:黔科合J字[2010]2202号),项目负责人:陈久毅~~

摘　　要：背景:作者前期研究表明,在临床运用中药石香膏治疗大量的软组织缺损、慢性难愈合创面取得了患者认可的疗效。目的:探讨中药石香膏促进软组织缺损修复的机制。方法:成年雄性新西兰大白兔30只,由贵州医科大学实验动物中心提供,实验方案经贵阳中医学院动物实验伦理委员会批准。对30只大白兔采用经典的全层皮肤切割伤造模,随机分为石香膏组、阳性对照组(贝复济组)和空白对照组,分别在创面上外敷石香膏+用敷料覆盖、外喷重组牛碱性成纤维细胞生长因子+用敷料覆盖、不含药物的干净敷料覆盖创面。分别于造模后第3,6,9,12天进行换药,观察创面的愈合情况、肉芽组织情况,记录创面愈合时间;收集新生肉芽组织标本,病理切片观察成纤维细胞数量、血管改变情况;观察各组创面碱性成纤维细胞生长因子及转化生长因子β1蛋白表达。结果与结论:①石香膏组与贝复济组创面愈合率高,愈合时间短,疗效优于空白组;②病理学观察石香膏组可见明显毛细血管增生,炎症反应轻,新生肉芽中可见许多成纤维细胞及炎性细胞浸润;在同一取材的条件下,造模后6,12d,3组转化生长因子β1、碱性成纤维细胞生长因子蛋白表达均显著高于造模3d(P <0.05);③结果表明,石香膏增加创面内源性生长因子转化生长因子β1和碱性成纤维细胞生长因子表达量,促进软组织缺损修复。BACKGROUND: Shixiang plaster has been shown to be effective for treating soft tissue defects and chronic healing.OBJECTIVE: To explore the mechanism of traditional Chinese medicine Shixiang plaster to promote the repair of soft tissue defects.METHODS: Thirty adult male rabbits were provided by Laboratory Animal Center of Guizhou Medical University, and the study was approved by the Experimental Animal Ethics Committee of Guiyang University of Traditional Chinese Medicine. All rabbits underwent classic full-thickness skin-cutting model, and then randomly divided into Shixiang plaster group(Shixiang plaster + dressing), Beifuji group(recombinant bovine basic fibroblast growth factor + dressing)and blank control group(dressing). Dressing change was performed at 3, 6, 9 and 12 days, respectively, and the wound healing and granulation tissue were observed, and the wound healing time was recorded. The specimens of newborn granulation tissue were collected to observe the number of fibroblasts and vascular changes by pathological section. The expression levels of basic fibroblast growth factor and transforming growth factor-β1 protein in wounds were detected.RESULTS AND CONCLUSION:(1) The wound healing rate in the Shixiang plaster and Beifuji group was high, the healing time was short,and the curative effect was better than that in the blank control group.(2) Pathological observation: Shixiang plaster group showed obvious capillary proliferation, mild inflammatory reaction, many fibroblasts and inflammatory cell infiltration in new granulation. The expression levels of transforming growth factor-β1 and basic fibroblast growth factor protein in wounds at 6 and 12 days after modeling under the same conditions in all groups were significantly higher than those at 3 days after modeling(P < 0.05).(3) These results suggest that Shixiang plaster can increase the expression levels of endogenous growth factors transforming growth factor-β1 and basic fibroblast growth factor in wounds and promote the repair of soft tissue defec

关 键 词：石香膏 软组织缺损修复 转化生长因子β1 碱性成纤维细胞生长因子 慢性难愈合创面 

分 类 号：R285.5[医药卫生—中药学]