活动性类风湿关节炎调节性T细胞程序性细胞死亡蛋白-1/程序性死亡配体1表达及与临床指标的相关性  

Expression of PD-1/PD-L1 on regulatory T cells of patients with active rheumatoid arthritis and its correlation with clinical parameters

作　　者：武加标 任敏[1] 成菊兰 许联红[2] 戚传平[2] WU Jia-biao;REN Min;CHENG Ju-lan;XU Lian-hong;QI Chuan-ping(Department of Rheumatology,Wujin Hospital Affiliated to Jiangsu University, Changzhou 213002, Jiangsu, China;Central Laboratory, Wujin Hospital Affiliated to Jiangsu University, Changzhou 213002, Jiangsu,China)

机构地区：[1]江苏大学附属武进医院风湿科,江苏常州213002 [2]江苏大学附属武进医院中心实验室,江苏常州213002

出　　处：《中华临床免疫和变态反应杂志》2019年第2期113-117,共5页Chinese Journal of Allergy & Clinical Immunology

基　　金：常州市武进区科技支撑计划项目(WS201415);江苏大学医学临床科技发展基金(JLY20140042)~~

摘　　要：目的探讨程序性细胞死亡蛋白-1/程序性死亡配体1(PD-1/PD-L1)在活动性类风湿关节炎(rheumatoid arthritis,RA)患者外周血调节性T细胞表面表达变化及临床意义。方法用流式细胞术分别检测62例活动期RA患者外周血CD4^+CD25^+T细胞和CD4^+CD25^-T细胞群PD-1和PD-L1的阳性率,并设立39例健康体检者作为健康对照组,分析PD-1/PD-L1与实验室各指标和病情活动性的相关性。结果 PD-1在24例高度活动组和38例中低度活动组RA患者CD4^+CD25^+T细胞的表达水平分别为[(2.27±0.56)%和(1.83±1.08)%],高于健康对照组(1.42±0.49)%,差异有统计学意义(P<0.01)。PD-L1在高度活动组和中低度活动组RA患者CD4^+CD25^+T细胞的表达水平分别为[(2.06±0.62)%和(1.83±0.63)%],亦高于健康对照组(0.80±0.43)%,与对照组相比差异有统计学意义(P<0.001)。PD-1在CD4^+CD25^+T细胞表达水平与DAS28呈正相关(r=0.477,P<0.01),PD-L1在CD4^+CD25^-T细胞表达水平与DAS28无相关性(r=0.078,P=0.549)。结论 PD-1/PD-L1在活动性RA患者调节性T细胞表达存在差异,其与DAS28相关性提示PD-1/PD-L1信号通路可经调节性T细胞调控参与RA的发生。Objective To investigate the significance of PD-1/PD-L1 expression on the surface of CD4^+CD25^+ T cells from peripheral blood of patients with rheumatoid arthritis (RA). Methods The positive rate of PD-1/PD-L1 on CD4^+CD25^+ T cells from peripheral blood of 62 cases of activity RA and 39 healthy human were detected by flow cytometry, and the correlation between them with the laboratory indexes and the activity of the disease was analyzed. Results The PD-1 expression levels of CD4^+CD25^+T cells in the 24 RA patients from high activity groups and 38 patients from moderate and low activity groups were (2.27±0.56)% and (1.83±1.08)%,respectively, while the level of healthy control group was (1.42±0.49)%, and there was significant difference between each group ( P <0.01).The PD-L1 expression levels in the RA patients from the high activity group and moderate and low activity groups activity group was (2.06±0.62)% and (1.83±0.63)%,respectively, higher than that of the healthy control group (0.80±0.43)%, and the difference was statistically significant ( P <0.001). The expression level of PD-1 in CD4^+CD25^+T cells was positively correlated with DAS28 ( r =0.477, P <0.01). Conclusions Our findings suggested that expression of PD-1/PD-L1 on regulatory T cells of patients with active RA was up-regulated and correlated with DAS28. Thus, it led to the fact that PD-1/PD-L1 pathway might participate in the pathogenesis of RA through Treg regulation.

关 键 词：类风湿关节炎 程序性细胞死亡蛋白-1 程序性死亡配体1 调节性T细胞 流式细胞术 

分 类 号：R593.22[医药卫生—内科学]