整合光散射信息的生物细胞相位成像系统  

Biological Cell Phase Imaging System Integrated with Optical Scattering Information

作　　者：季颖[1] 傅爽 陶兆禾 梁敏捷 王亚伟[1] Ji Ying;Fu Shuang;Tao Zhaohe;Liang Minjie;Wang Yawei(Faculty of Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China;School of Mechanical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China)

机构地区：[1]江苏大学理学院,江苏镇江212013 [2]江苏大学机械工程学院,江苏镇江212013

出　　处：《激光与光电子学进展》2019年第9期194-202,共9页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(11474134);江苏省六大人才高峰(2015-DZXX-023)

摘　　要：提出了一种可将光散射信息整合到干涉相位成像系统的设计方法。建立了均质细胞、单核细胞、双核细胞模型,通过分析样品的相位分布信息快速定量提取样品的形态特征,确定了样品的亚结构特征。通过红细胞和中性粒细胞实验,指出了结合散射信息的必要性和优势。研究结果验证了所提系统的可行性,并提出了改进方向。该系统结构简单、便于操作,为细胞形态结构特征的快速无损检测提供了参考。A design method for an interferometric phase imaging system integrated with optical scattering information is proposed. Three types of cell models, i.e., homogeneous cells, monocytes, and binuclear cell models, are established. The morphological characteristics of a sample can be extracted quickly and quantitatively by analyzing the phase distribution information of the sample. Thus, the substructure characteristics of the sample can be determined accurately. The necessity and advantages of combining scattering information are demonstrated through experiments on the red blood cells and neutrophils. The research results establish the feasibility of the proposed system. Additionally, the direction for improvement is proposed. The system has a simple structure and is easy to operate, and it can provide a reference for the rapid, non-destructive detection of morphological and structural characteristics of cells.

关 键 词：生物光学 形态 相位成像系统 散射 生物细胞 

分 类 号：O436.1[机械工程—光学工程]