Compound 48/80诱导BN大鼠类过敏反应的蛋白组学研究  

Proteomic study of Compound 48/80 induced anaphylactoid reaction in BN rats

作　　者：陈桂荣[1] 徐煜彬 冉小库[1] 韩雪莹[1] 窦德强[1] CHEN Gui-Rong;XU Yu-Bin;RAN Xiao-Ku;HAN Xue-Ying;DOU De-Qiang(College of Pharmacy,Liaoning University of Traditional Medicine,Dalian 116600,China;Taizhou Central Hospital (Taizhou University Hospital),Taizhou 318000,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]台州市中心医院台州学院附属医院,浙江台州318000

出　　处：《现代药物与临床》2019年第5期1279-1284,共6页Drugs & Clinic

基　　金：国家自然科学基金青年科学基金资助项目(81303205);辽宁省高等学校创新人才计划项目(LR2017002);沈阳市中青年科技创新人才支持计划项目(RC170344)

摘　　要：目的探讨Compound 48/80诱导BN大鼠类过敏反应的发生机制,采用iTRAQ蛋白组学技术对类过敏的发生过程进行研究,并鉴定候选标志物。方法将BN大鼠按体质量随机分为对照1组、2组和Compound 48/80 1组、2组,每组各6只。对照组iv生理盐水5 mL/kg,Compound 48/80组分别iv Compound 48/80 2.5 mL/kg,取血浆样品经蛋白提取、电泳和质谱分析,确定类过敏反应差异蛋白。结果通过蛋白组学研究共鉴定10585个肽段,总共鉴定1987个蛋白质。提取7个与类过敏反应相关的差异蛋白。与对照组比较,Ica、Galectin-1上调;Hpse HEP、Mpo、MMP8、Hprt1、MMP9下调。结论Hpse HEP、Mpo、MMP8、Hprt1、Ica、MMP9、Galectin-1均可以作为类过敏反应的候选生物标志物。Objective To investigate the mechanism of Compound 48/80-induced anaphylactoid reaction in BN rats, and ITRAQ proteomics technique was used to study the occurrence of anaphylaxis, and identify candidate markers. Methods BN rats were randomly divided into control group 1 and 2, Compound 48/80 group 1 and 2 according to body weight, and each group had 6 rats.Rats in control group were iv administered with normal saline 5 mL/kg, and Compound 48/80 groups were iv Compound 48/802.5 mL/kg. The plasma samples were analyzed by protein extraction, electrophoresis, and mass spectrometry to determine the differential proteins of anaphylactoid reaction. Methods A total of 10 585 peptide segments and 1 987 proteins were identified by proteomics. Seven differential proteins related to anaphylactoid reaction were extracted. Compared with the control group, Ica and Galectin-1 were up-regulated, and Hpse HEP, Mpo, MMP8, Hprt1, and MMP9 were down-regulated. Conclusion Hpse HEP, Mpo,MMP8, Hprt1, Ica, MMP9, and Galectin-1 can be used as candidate biomarkers for anaphylaxis.

关 键 词：Compound48/80 类过敏反应 蛋白组学 差异蛋白 

分 类 号：R967[医药卫生—药理学]