大鼠心肌细胞和心肌细胞系H9C2表达弹性蛋白  

Expression of elastin in rat cardiomyocytes and cardiomyocyte cell line H9C2

作　　者：韩贝 任明芬[2] 常玉巧[1] 郭志坤[1] HAN Bei;REN Ming-fen;CHANG Yu-qiao;GUO Zhi-kun(He'nan Key Laboratory of Medical Tissue Regeneration;Cardiovascular Department, the Second Affiliated Hospital, Xinxiang Medial University He'nan Xinxiang 453003, China)

机构地区：[1]新乡医学院河南省医用组织再生重点实验室,河南新乡453003 [2]新乡医学院第二附属医院心血管内科,河南新乡453000

出　　处：《解剖学报》2019年第3期292-296,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金(81570268);河南省科技攻关项目(152102310111)

摘　　要：目的检测弹性蛋白在大鼠心肌细胞和H9C2心肌细胞系内的表达。方法取新生和成年SD大鼠各5例的新鲜心脏,冷冻切片;取20只新生3 d SD大鼠心脏,通过0.05%胰蛋白酶和0.075%Ⅱ型胶原酶消化法,获取原代心肌细胞;免疫组织化学和免疫荧光技术检测大鼠心肌组织、原代心肌细胞和H9C2心肌细胞系的弹性蛋白表达;ELISA法检测培养的H9C2心肌细胞系上清中的弹性蛋白。结果在大鼠心肌细胞、H9C2心肌细胞系和新生、成年大鼠心肌组织上均发现心肌肌钙蛋白T和弹性蛋白的共表达。免疫组织化学结果发现,弹性蛋白在成纤维细胞、平滑肌细胞和细胞间质内均有表达。H9C2心肌细胞培养液的上清中检测到少量的弹性蛋白。结论大鼠心肌细胞表达弹性蛋白,可能参与心肌细胞的势能和细胞间质弹性纤维的构建。Objective To detect the expression of elastin in cardiomyocytes and cardiomyocyte cell line H9 C2. Methods The five fresh hearts of newborn and adult SD rats are made into frozen slices.Primary cardiomyocytes were isolated from the hearts of the twenty neonatal 3 days SD rats by the digestion of 0.05% trypsin and 0.075% type Ⅱ collagenase.The technology of immunochemical and immunofluorescence were used to detect the expression of elastin in rat myocardial tissue, primary cardiomyocytes and cardiomyocyte cell line H9 C2.Elastin in supernatant of cultured cardiomyocyte cell line H9 C2 was detected by ELISA. Results The co-expression of cardiac troponin T and elastin was found in rat cardiomyocytes, cardiomyocyte cell line H9 C2,neonatal and adult SD rat myocardial tissues.The result of immunohistochemistry also revealed the expression of elastin in fibroblasts, smooth muscle cells, and interstitial substance.A small amount of elastin was detected in the supernatant of cardiomyocyte cell line H9 C2. Conclusion Rat cardiomyocytes express elastin,it may be involved in the potential energy of myocardial cells and the construction of intercellular elastic fibers.

关 键 词：弹性蛋白 心肌细胞 心肌细胞系H9C2 免疫组织化学 免疫荧光 酶联免疫吸附测定 大鼠 

分 类 号：Q28[生物学—细胞生物学]