丹参类黄酮3-O糖基转移酶基因Sm UF3GT的克隆、亚细胞定位及时空表达分析  

Cloning,subcellular localization and spatio-temporal expression analysis of a flavonoid 3-O-glucosyltransferase gene( SmUF3GT) in Salvia miltiorrhiza

作　　者：李红艳[1] 刘景玲[1] 金伟波[3] 梁宗锁[1,2,3] LI Hong-yan;LIU Jing-ling;JIN Wei-bo;LIANG Zong-suo(College of Life Science, Northwest Agriculture and Forestry University, Yangling 712100, China;Institute of Soil and Water Conservation, Chinese Academy of Sciences and the Ministry of Water Resources, Yangling 712100, China;College of Ltfe Science, Zhejiang Sci-Tech University, Hangzhou 310018, China)

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]中国科学院水利部水土保持研究所,陕西杨凌712100 [3]浙江理工大学生命科学学院,浙江杭州310018

出　　处：《中国中药杂志》2019年第10期2038-2045,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81778385);国家中医药管理局标准化项目(ZYBZH-C-TJ-55)

摘　　要：类黄酮3-O糖基转移酶家族(UFGT)是植物细胞中催化不稳定花青素转化成稳定花青素苷的关键酶。该研究基于同源克隆和转录组数据库从丹参花瓣中克隆得到了UFGT家族基因之一,命名为Sm UF3GT。该基因包含一个1 353 bp的开放阅读框,编码450个氨基酸,并进行了生物学信息分析。初步定位于细胞质膜,细胞核,以及细胞基质中的过氧化物酶体、高尔基体上。qRT-PCR分析表明,Sm UF3GT在丹参和白花丹参中除了根的其他组织器官均有不同丰度的表达;在花发育进程中,在丹参花瓣中的表达呈现先升后降的趋势,而在白花丹参花瓣中呈现先急剧下降后缓慢下降的趋势。该研究为进一步探讨Sm UF3GT基因在丹参类黄酮次生代谢产物的合成积累机制中的作用奠定基础。The family of flavonoid 3-O-glucosyltransferase catalyzes the modification of anthocyanin from unstable-structure to stable-structure. In this study,based on homology cloning and transcriptome library,we isolated the full-length c DNA of UDP-glucose: flavonoid 3-O-glucosyltransferase( named SmUF3GT) from the flower tissues of S. miltiorrhiza. This gene was consisted of 1 353 bp open reading frames( ORF) encoding 450 amino acids. And the SmUF3GT protein was performed for the bioinformatic analysis. Our results showed that the protein was preliminary localized in the Golgi and peroxisome of cytosol,as well as plasma membrane and cell nuclear.QRT-PCR analyses indicated that SmUF3GT expressed differently in all tissues and organs but roots of S. miltiorrhiza and S. miltiorrhiza f.alba. During floral development,the expression of SmUF3GT showed a trend of rising fist and then down in purple-flower Danshen,whereas decreasing sharply fist and then slowly in white-flower Danshen. The present study provides basic information for further research on the network of synthesis and accumulation of flavonoids in S.miltiorrhiza.

关 键 词：丹参 SmUF3GT 白花丹参 亚细胞定位 实时荧光定量 时空表达模式 

分 类 号：S567.53[农业科学—中草药栽培]