扫描离子电导显微镜在细胞表征中的应用研究  

Application of scanning ion conductance microscope in cell characterizations

作　　者：郎瑾新 李亚北 杨耀维 焦阳博翰 王蒙[2,3] 庄健 李菲[2,3] Jinxin Lang;Yabei Li;Yaowei Yang;Yangbohan Jiao;Meng Wang;Jian Zhuang;Fei Li(Department of Chemistry, Xi'an Jiaotong University, Xi'an 710049, China;Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China;The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China;School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China)

机构地区：[1]西安交通大学理学院化学系,西安710049 [2]西安交通大学仿生工程与生物力学中心(BEBC),西安710049 [3]西安交通大学生命科学与技术学院,生物医学信息工程教育部重点实验室,西安710049 [4]西安交通大学机械工程学院,西安710049

出　　处：《中国科学：化学》2019年第6期844-860,共17页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21775117);2017年度陕西省留学人员科技活动择优资助优秀项目(编号:2017010);陕西省国际科技合作与交流计划项目(编号:2016KW-064);中国博士后科学基金面上项目(编号:2016M592773);西安交通大学基本科研业务费重点研发计划专项培育类项目资助

摘　　要：细胞的微观形貌、电学和力学功能在细胞生物学研究中具有重要意义.但传统细胞表征方法无法实现对活体细胞微/纳观尺度的无损、原位表征.扫描离子电导显微镜(scanning ion conductance microscopy, SICM)作为新型的扫描探针显微镜技术,由于非接触式扫描模式及纳米探针的使用,可实现对活细胞无损、高分辨地实时表征,近年来被广泛用于各种细胞生物学和细胞表征研究中.本综述主要介绍了SICM的发展历史及其在细胞表征中的应用.首先介绍SICM的仪器组成和工作原理、三种常见的工作模式及优缺点,之后分类介绍SICM在细胞生物学领域的应用进展,包括SICM在细胞形貌表征、细胞电学性质和力学性质研究中的具体应用实例,总结和比较了SICM与传统细胞生物学表征方法在细胞原位表征中的优势.最后,提出SICM在细胞表征方面面临的挑战,对其未来技术发展方向进行了展望.Cell morphology, electrical and mechanical functions are of great significance in cell biology. However,traditional cell characterization methods are not suitable for study of living cells in a non-invasive, in situ and micro/nano-scaled way. As a new type of scanning probe microscopy, scanning ion conductance microscopy(SICM) enables non-invasive, high-resolution and real-time imaging of living cells based on its non-contact scanning mode and usage of nanopipette as its probe. In recent years, SICM has been widely applied in cell biology and cell characterizations. This review mainly introduces the SICM applications in cell characterizations. First, we introduce the instrumental composition, working principle and three work modes of SICM, and compare the strengths and weakness of the three working modes. Then the applications of SICM in cell morphology, cellular electrical and mechanical properties are described in detail. In addition, in contrast to the traditional cell characterization methods, SICM has the distinct advantages in in situ characterization of cells. Finally, the challenges of SICM in cell characterizations are discussed and its future development is proposed.

关 键 词：扫描离子电导显微镜 细胞形貌 细胞电学 细胞力学 

分 类 号：Q2-33[生物学—细胞生物学]