不同种质地黄块根菊花心表型性状及HPLC指纹图谱特征分析  

Phenotypic Character and HPLC Fingerprints of Radial Striations from Different Germplasms Rehmanniae Radix

作　　者：朱建光[1] 王丰青[2] 谢彩侠[1,3] 郭晓玉 张苗[1] 陶晓赛[1] 耿晓桐 雷敬卫 张重义[3] ZHU Jian-guang;WANG Feng-qing;XIE Cai-xia;GUO Xiao-yu;ZHANG Miao;TAO Xiao-sai;GENG Xiao-tong;LEI Jing-wei;ZHANG Zhong-yi(Henan University of Chinese Medicine, Zhengzhou 450046,China;Henan Agricultural University, Zhengzhou 450002,China;Fujian Agriculture and Forestry University, Fuzhou 350021,China)

机构地区：[1]河南中医药大学,郑州450046 [2]河南农业大学,郑州450002 [3]福建农林大学,福州350021

出　　处：《中国实验方剂学杂志》2019年第12期166-173,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点研发计划项目(2017YFC1700705);河南省科技攻关项目(182102311156)

摘　　要：目的:探讨不同种质地黄块根菊花心表型性状及高效液相(HPLC)指纹图谱特征。方法:观察测量地黄整个生育期内菊花心形状及比例直径的变化,同时建立表征地黄块根、菊花心及非菊花心部位化学质量的HPLC指纹图谱,并采用主成分和系统性聚类方法对其进行分析。结果:不同种质地黄的菊花心形状及大小存在明显差异且具有一定的规律性;指纹图谱显示不同种质地黄不同部位的化学成分种类具有一致性,其化学质量特征的差异主要在于化学成分含量及其相对比例的不同;主成分分析结果表明毛蕊花糖苷、梓醇、益母草苷、地黄苷A和地黄苷D等有效成分基本均参与了各种质地黄不同部位的质量表达,但每种成分在各种质地黄不同部位之间质量差异表达中的贡献率不同,同时参与这种差异表达的其他成分在各种质地黄中的贡献率也不同;系统性聚类分析表明,北京1号,沁怀,1706及沁怀郑地黄的菊花心与非菊花心化学质量特征差异较大,而85-5,白选地黄的菊花心与非菊花心部位化学质量特征差异较小。结论:不同种质地黄菊花心表型性状及其HPLC指纹图谱特征具有一定的差异。Objective: To discuss the phenotypic character and the HPLC fingerprints of radial striations from different germplasms Rehmanniae Radix. Method: The changes in the shape and column diameter of the radial striations of Rehmanniae Radix were observed and measured in the whole growth period. Besides,the HPLC fingerprints of the root,radial and un-radial striations were established to sign the chemical quality and analyzed by principal component analysis( PCA) and systematic cluster analysis. Result: There were significantly differences and regularities in the shape and proportion of the radial striations of different germplasms Rehmanniae Radix. The fingerprints showed the consistency between different types of chemical ingredients,and the differences in chemical quality characteristics mainly lay in the content of chemical compositions and theirs relative ratio. The results of PCA indicated that active ingredients,such as acteoside, catalpol, rehmaionoside D, rehmaionoside A and leonuride,were involved in the quality expression of different parts from various germplasms of Rehmanniae Radix,but each ingredient had a distinctive contribution rate to the differential quality expression between different parts from various germplasms of Rehmanniae Radix. However,the other components involved in the differential quality expression had different contribution rates in different germplasms. The systematic cluster analysis indicated that great differences in the chemical quality between the radial striations and un-radial striations of Beijing-1,Qinhuai,Qinhuai Zheng and 1706 germplasms,but with small differences in 85-5 and Baixuan germplasms. Conclusion:There are differences in phenotypic character of the radial striations and HPLC fingerprints between different germplasms Rehmanniae Radix.

关 键 词：地黄 菊花心 表型性状 高效液相色谱指纹图谱 主成分分析 系统性聚类分析 

分 类 号：R284.1[医药卫生—中药学] R281[医药卫生—中医学]