珍珠透骨草HPLC指纹图谱及抗炎活性的谱效关系  

Correlation Analysis Between HPLC Fingerprint and Anti-inflammatory Activity of Speranskiae Tuberculatae Herba

作　　者：申鸽 景大山 杨飞[1] 陈婕 葛睿[1] 杨官娥[1] SHEN Ge;JING Da-shan;YANG Fei;CHEN Jie;GE Rui;YANG Guan-e(School of Pharmacy, Shanxi Medical University, Taiyuan 030001,China)

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《中国实验方剂学杂志》2019年第12期174-180,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省中药材中药饮片标准研究项目(2016010C;2016003C);国家自然科学基金项目(30672621;81173473);山西省自然科学基金项目(2010011048-2);太原市科技项目(120247-08)

摘　　要：目的:建立珍珠透骨草药材的HPLC指纹图谱,并研究其与抗炎活性之间的谱效关系。方法:采用HPLC法建立珍珠透骨草的指纹图谱;在相似度评价基础上,结合聚类分析(HCA)和主成分分析(PCA),对其中的共有峰进行评价;建立二甲苯致小鼠耳肿胀模型考察珍珠透骨草的抗炎活性,灰色关联度分析(GRA)和偏最小二乘回归分析(PLSR)研究谱效关系。结果:建立了珍珠透骨草的HPLC指纹图谱,确定了其中24个色谱峰为共有峰,相似度除了S2和S5,均在0. 907以上,通过对照品比对法指认了其中4个峰。HCA结果表明,全部样品被聚为四类,与PCA结果基本一致,结合偏最小二乘法判别分析(PLS-DA)发现,5,6和7号峰为造成珍珠透骨草样品组间差异的3个标志性化合物。不同来源的珍珠透骨草药材均有不同程度的抗炎活性,谱效关系表明,1,4,5,6和10号峰是与抗炎活性关联较大的正相关峰。结论:建立了珍珠透骨草药材的HPLC指纹图谱,并确定1,4,5,6和10号峰为5个与抗炎活性密切相关的成分,为药材质量控制提供更全面的参考。Objective: The aim of this study was to investigate high performance liquid chromatography( HPLC) fingerprint, anti-inflammatory activity as well as the correlation between them in Speranskiae Tuberculatae Herba. Method: Fingerprint by HPLC was established on Speranskiae Tuberculatae Herba from different sources. The common peaks were evaluated on the basis of similarity evaluation together with hierarchical cluster analysis( HCA) and principal component analysis( PCA). Ear swelling induced by xylene in mice model was used to study the anti-inflammatory activity. Grey relational analysis( GRA) and partial least square regression analysis( PLSR) were used to study the relationship between the HPLC fingerprint and the anti-inflammatory activity. Result: The HPLC fingerprint of Speranskiae Tuberculatae Herba was established and 24 common peaks were determined, with the similarity above 0. 907( except for S2 and S5). Four peaks were identified by comparing to reference substances. The result of HCA showed that Speranskiae Tuberculatae Herba from different sources were clustered into four categories,in consistent with the result of PCA. Combined with discriminant analysis of partial least squares discrimination analysis( PLS-DA),three signature compounds represented by 5,6,7 peaks were responsible for the differences between groups. Different sources of Speranskiae Tuberculatae Herba differed in anti-inflammatory activity. The sectrum-activity relationship showed that the peaks 1,4,5,6,and 10 were positively correlated with the anti-inflammatory activity. Conclusion: The HPLC fingerprint of Speranskiae Tuberculatae Herba was established,and 5 components closely related to the anti-inflammatory activity were determined. The present study provide more comprehensive reference for quality control of the Speranskiae Tuberculatae Herba.

关 键 词：珍珠透骨草 指纹图谱 抗炎活性 谱效关系 灰色关联度分析 偏最小二乘回归分析 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]