模拟微重力对人淋巴母细胞TK6 lncRNA和mRNA表达的影响  

Effect of simulated microgravity on expressions of lncRNA and mRNA in TK6 human lymphoblastoid cells

作　　者：杨玫 朱捷 郑晓飞 李清 付汉江 YANG Mei;ZHU Jie;ZHENG Xiao-fei;LI Qing;FU Han-jiang(College of Life Science and Technology,Dalian University,Dalian,Liaoning 116000,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Academic Affairs Division,Dalian University,Dalian,Liaoning 116000,China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116000 [2]军事科学院军事医学研究院辐射医学研究所,北京100850 [3]大连大学教务处,辽宁大连116000

出　　处：《军事医学》2019年第1期20-24,共5页Military Medical Sciences

基　　金：国家自然科学基金(81773038);全军后勤科研资助项目(BEP16001)

摘　　要：目的了解微重力对mRNA及长链非编码RNA(lncRNA)表达的影响,以及它们在微重力条件下的作用。方法通过对人淋巴母细胞TK6在静态或旋转以模拟微重力环境进行培养,用基因芯片及生物信息学方法分析lncRNA及mRNA表达谱,以及微重力对它们的调控作用。结果芯片结果显示有数十个基因表达发生显著改变,大部分表达下调,这些表达下调基因的功能多与胆固醇合成关系密切。定量PCR验证结果显示,在模拟微重力处理24 h后,有1个lncRNA和8个mRNA的表达发生了显著下调。处理48 h后,有1个lncRNA及14个mRNA表达下调,且表达下降更为显著。结论模拟微重力能调控TK6细胞中lncRNA和mRNA的表达,这些基因的表达变化可能与微重力给生命机体带来的影响相关。Objective To analyze the effects of microgravity on the expressions of mRNAs and long noncoding RNAs(lncRNAs),and the role of these RNA molecules in the microgravity environment. Methods TK6 cells were cultured in a static or rotating manner to simulate the microgravity environment,and the effect of microgravity on lncRNAs and mRNAs expressions was analyzed via microarray and bioinformatics. Results The results of the microarray showed that there were dozens of gene expressions that had changed significantly,most of which were down-regulated,and that the functions of these down-regulated genes were closely related to cholesterol synthesis. The results of quantitative PCR showed that the expressions of 1 lncRNA and 8 mRNAs decreased significantly after 24 h of simulated microgravity treatment. After 48 h treatment,1 lncRNA and 14 mRNAs were down-regulated,and the expressions of these genes decreased more significantly.Conclusion Simulated microgravity can regulate the expressions of lncRNA and mRNA in TK6 cells,and the expression changes in these genes may be related to the effects of microgravity on living organisms.

关 键 词：长链非编码RNA RNA 信使 微重力 失重模拟 基因表达 肿瘤坏死因子Α 白细胞介素类 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]