电针“足三里”或“阳陵泉”对围产期尼古丁暴露新生鼠肺功能和组织形态的影响  

Effect of electroacupuncture at “Zusanli”(ST 36) and “Yanglingquan”(GB 34) on perinatal nicotine-exposure-induced lung function and morphology of neonatal rats

作　　者：苏杭 嵇波[1] 赵国桢[1] 刘翼天 葛云鹏 戴健[1] 路雅雯 王丹[1] Reiko Sakurai Rehan VK 严明娜 孙晓敏[1] 白红新[1] 郭孟玮[1] 任晓暄[1] SU Hang;JI Bo;ZHAO Guo-zhen;LIU Yi-tian;GE Yun-peng;DAI Jian;LU Ya-wen;WANG Dan;YAN Ming-na;SUN Xiao-min;BAI Hong-xin;GUO Meng-wei;REN Xiao-xuan(School of Acupuncture,Moxibustion and Tuina,Beijing University of CM,Beijing 100029,China;Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center,Los Angeles 90502,USA)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]加州大学洛杉矶分校医疗中心生物医学研究所,洛杉矶90502

出　　处：《中国针灸》2019年第6期632-636,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金面上项目:81674059;81373558;北京中医药大学在读研究生项目:2018-JYB-XS

摘　　要：目的:对比电针母鼠"足三里"与"阳陵泉"穴对围产期尼古丁暴露新生鼠肺功能与组织形态学干预效应的差异,探寻电针防护新生鼠肺发育不良的穴位效应规律。方法:将24只雌性SD大鼠随机分为生理盐水组(S组)、尼古丁组(N组)、尼古丁加足三里组(N+ST 36组)和尼古丁加阳陵泉组(N+GB 34组),每组6只。从受孕第6天开始,S组皮下注射0.9%氯化钠溶液(1 mg/kg);其余3组皮下注射等量尼古丁,至子代大鼠出生后第21天制备围产期尼古丁暴露模型。造模同时N+ST 36组和N+GB 34组分别电针"足三里"和"阳陵泉"穴,每天1次,至子代大鼠出生后第21天。采用小动物肺功能分析系统观察新生鼠吸气峰值流速(PIF)、呼气峰值流速(PEF)、呼气阻力(RE)、吸气阻力(RI)和动态顺应性(Cdyn)等肺功能指标变化;采用HE染色观察肺泡融合、破裂程度等肺组织形态学的变化。结果:与S组比较,N组PEF、Cdyn较低,PIF、RI、RE较高(均P<0.01),肺泡融合、破裂,肺泡壁增厚,肺泡数量减少,肺泡间距变大,肺泡直径较大(P<0.01);与N组比较,N+ST 36组PEF、Cdyn较高,PIF、RI、RE较低(P<0.05,P<0.01),肺泡融合、破裂程度有所改善,肺泡数量增多,肺泡壁变薄,肺泡间距正常,肺泡直径显著减少(P<0.01),而N+GB34组肺功能和肺形态各指标变化不明显(P>0.05)。结论:电针围产期尼古丁暴露孕鼠"足三里"较"阳陵泉"更能明显改善新生大鼠肺功能与肺组织形态。Objective To compare the effects of electroacupuncture(EA) at "Zusanli"(ST 36) versus "Yanglingquan"(GB 34) in the pregnant rats on perinatal nicotine-exposure-induced lung function and morphology of newborn rats and explore the rule of acupoint effect in EA for the prevention from lung dysplasia in newborn rats. Methods A total of 24 female SD rats were randomized into a normal saline group(S group), a nicotine group(N group), a nicotine-ST 36 group(N + ST 36 group) and a nicotine-GB 34 group(N+GB 34 group), 6 rats in each one. Starting at the 6 th day of pregnancy, 0.9% sodium chloride solution was injected subcutaneously in the S group, 1 mg/kg;and in the rest 3 groups, nicotine of the same dose was injected through to the 21 st postnatal day to establish the perinatal nicotine-exposure model. Simultaneously, during model preparation, EA was applied at "Zusanli"(ST 36) and "Yanglingquan"(GB 34) in the N+ST 36 group and the N+GB 34 group respectively, once a day, through to the 21 st postnatal day. The lung function analytic system for small animal was adopted to observe the changes in lung function indicators in newborn rats, such as peak inspiratory flow(PIF), peak expiratory flow(PEF), expiratory resistance(RE), inspiratory resistance(RI) and dynamic compliance(Cdyn). HE staining was used to observe the morphological changes of lung, such as alveolar fusion and rupture. Results Compared with the S group, PEF and Cdyn were lower and PIF, RI and RE higher in the N group(all P<0.01), additionally, alveoli were fused and ruptured, alveolar wall thickened, the numbers of alveoli reduced, the interspace of alveoli enlarged and the diameter increased(P<0.01). Compared with the N group, in the N+ST 36 group, PEF and Cdyn were increased, PIF, RI and RE reduced(P<0.05, P<0.01), the alveolar fusion and rupture relieved, the numbers of alveoli increased, alveolar wall thinner, the interpsace of alveoli became normal and the diameter was reduced significantly(P<0.01). In the N+GB 34 group, the changes of lung function an

关 键 词：围产期尼古丁暴露 电针 肺发育 肺功能 肺形态 

分 类 号：R245[医药卫生—针灸推拿学]