镧对人参发根中皂苷积累及合成关键酶基因表达的影响  

Effects of Lanthanum on Accumulation of Ginsenosides and Expression of Key Enzymes Genes of Ginsenosides Biosynthesis in Hairy Roots of Panax Ginseng

作　　者：张儒[1] 张变玲[1] 黄雪梅 崔甜 李贵[2,3] 罗志勇[2] Zhang Ru;Zhang Bianling;Huang Xuemei;Cui Tian;Li Gui;Luo Zhiyong(College of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104 , China;Molecular Biology Research Center, School of Ufa Sciences, Central South University, Changsha410078, China;ollege of Biological Resources and Environment Science, Jishou University,Jishou 416000, China)

机构地区：[1]湖南工程学院化学化工学院,湖南湘潭411104 [2]中南大学生命科学学院分子生物学研究中心,湖南长沙410078 [3]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《中国稀土学报》2019年第3期361-370,I0004,共11页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金项目(81874332,81673544);湖南省自然科学基金项目(2017JJ3048,2016JJ2037);湖南省教育厅基金项目(18B387,16C0394);中国博士后特别资助和面上基金项目(2017T100601,2016M590746);国家级大学生创新训练计划项目(201811342007)资助

摘　　要：以不同浓度镧处理人参发根, qRT-PCR检测人参皂苷合成关键酶基因PgSS,PgDDS和PgβAS的表达水平, HPLC测定人参皂苷含量,探讨镧对人参发根生长及其人参皂苷合成影响的作用机制。镧浓度分别为5.0, 10.0和15.0 mg·L^-1时,促进发根伸长、根毛形成和重量增加, 20.0, 25.0和30.0 mg·L^-1时,抑制发根生长;PgSS和PgDDS基因表达水平对镧呈浓度依赖性变化, 20.0 mg·L^-1时,PgSS和PgDDS基因表达量均显著增加(P<0.05)且达到最大,高于20.0 mg·L^-1时,PgSS和PgDDS基因表达水平随镧浓度升高而降低;镧对PgβAS基因表达无明显影响。镧促进发根中人参皂苷Rb1, Rb2, Rc, Rd, Re, Rg1和总皂苷含量增加,人参皂苷合成积累规律与PgSS和PgDDS基因表达规律一致,当镧浓度为20.0 mg·L^-1时,人参皂苷产量亦达到最高。表明镧可以调节人参发根的生长,通过促进人参皂苷合成关键酶基因PgSS和PgDDS的表达增加人参皂苷的生物合成与积累。To investigate the effect of lanthanum(La) on the ginsenosides biosynthesis and its key enzymes genes expression in hairy roots of Panax ginseng. La was added to the media of ginseng hairy roots with 0.0, 5.0, 10.0, 15.0, 20.0, 25.0 and 30.0 mg·L^-1, respectively. The expression levels of PgSS, PgDDS and PgβAS genes were identified by qRT-PCR, and the content of ginsenoside Rb1, Rb2, Rc, Rd, Re, Rg1 and total saponins was assayed by HPLC. The correlation between expression profiles of key enzymes genes and the ginsenosides biosynthesis and accumulation was also analyzed. The results indicated that La enhances the extension, root hair formation and biomass of ginseng hairy roots at 5.0, 10.0 and 15.0 mg·L^-1, and the growth of hairy roots is inhibited by La at 20.0, 25.0 and 30 mg·L^-1. PgSS and PgDDS genes expression is induced by La in a concentration dependent way, and the expression level increases obviously(P<0.05) and reaches the highest level at 20.0 mg·L^-1, and then decreases. However, La does not affect the PgβAS gene. At the meantime, biosynthesis and accumulation of ginsenoside Rb1, Rb2, Rc, Rd, Re, Rg1, total saponins increase obviously in a same way of the expression patterns of PgSS and PgDDS genes, the yeild of ginsenosides reaches to the climax at 20.0 mg·L^-1 La. In conclusion, La can improve the expression of PgSS and PgDDS genes and its corresponding metabolites ginsenoside in hairy roots of P. ginseng. This work provides a foundation for investigation of the accumulation mechanism of ginsenosides under the control of La in P. ginseng.

关 键 词：人参发根 镧处理 人参皂苷 关键酶 基因表达 

分 类 号：S567.51[农业科学—中草药栽培]