A Single-Cell RNA Sequencing Profiles the Developmental Landscape of Arabidopsis Root  

作　　者：Tian-Qi Zhang Zhou-Geng Xu Guan-Dong Shang Jia-Wei Wang 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics(NKLPMG),CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology(SIPPE),Shanghai 200032,P.R.China [2]University of Chinese Academy of Sciences,Shanghai 200032,P.R.China [3]ShanghaiTech University,Shanghai 200031,P.R.China

出　　处：《Molecular Plant》2019年第5期648-660,共13页分子植物（英文版）

基　　金：the State Key Basic Research Program of China(2013CB127000);National Natural Science Foundation of China(31430013;31222029;912173023,31525004);Strategic Priority Research Program of the Chinese Academy of Sciences(XDB27030101);Young Elite Scientists Sponsorship Program by CAST(2016QNRC001);National Postdoctoral Program for Innovative Talents(BX201600178);the Sanofi-SI BS 2017 Post-doctoral Fellowship.

摘　　要：Cells of eukaryotic multicellular organisms have inherent heterogeneity.Recent advances in single-cell gene expression studies enable us to explore transcriptional regulation in dynamic development processes and highly heterogeneous cell populations.In this study,using a high-throughput single-cell RNA-sequencing assay,we found that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes.A total of 24 putative cell clusters and the cluster-specific marker genes were identified.The spatial distribution and temporal ordering of the individual cells at different developmental stages illustrate their hierarchical structures and enable the reconstruction of continuous differentiation trajectory of root development.Moreover,we found that each root cell cluster exhibits distinct patterns of ion assimilation and hormonal responses.Collectively,our study reveals a high degree of heterogeneity of root cells and identifies the expression signatures of intermediate states during root cell differentiation at single-cell resolution.We also established a web server(http://wanglab.sippe.ac.cn/rootatlas/)to facilitate the use of the datasets generated in this study.

关 键 词：ARABIDOPSIS ROOT scRNA-seq cell ATLAS differentiation trajectory MERISTEM 

分 类 号：Q[生物学]