艾灸“神阙”“足三里”穴对痴呆大鼠脂联素及受体表达的影响  

Effect of moxibustion at “Shenque”(CV8) and “Zusanli”(ST36) on expression of adiponectin and adiponectin receptor in Alzheimer’s disease rats

作　　者：赵彩娇 黄逸珠 蒋云芳 卢美静 ZHAO Cai-jiao;HUANG Yi-zhu;JIANG Yun-fang;LU Mei-jing(School of Acupuncture-moxibustion and Massage,Guangxi University of Traditional Chinese Medicine, Nanning 530001,China)

机构地区：[1]广西中医药大学针灸推拿学院

出　　处：《针刺研究》2019年第6期419-423,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81260528);广西中医药大学研究生教育创新计划项目(No.YCSZ2018008)

摘　　要：目的:通过比较艾灸不同腧穴对阿尔茨海默病(AD)大鼠血清及脂肪组织中脂联素及脂联素受体的影响,探讨艾灸“神阙”“足三里”对AD大鼠脂质代谢的作用机制。方法:SD大鼠随机分为空白组、模型组、神阙组、足三里组、神阙-足三里组,每组10只。大鼠腹腔注射D-半乳糖、氢溴酸东莨菪碱复制AD模型,各治疗组艾灸相应腧穴,每日治疗1次,每次每穴艾灸3壮,连续5周。用Morris水迷宫定位航行实验检测大鼠记忆能力,用酶联免疫吸附法检测大鼠血清中脂联素的水平,用荧光定量PCR和Western blot法分别检测大鼠脂肪组织中脂联素mRNA及脂联素受体的表达。结果:与正常组比较,模型组大鼠首次水迷宫定位航行实验的逃避潜伏期显著延长,说明造模成功。干预结束后,与正常组比较,模型组大鼠血清中脂联素含量显著降低(P<0.01),脂肪组织中脂联素mRNA显著降低(P<0.01),脂肪组织中脂联素受体表达显著下降(P<0.01)。与模型组比较,各艾灸组大鼠逃避潜伏期显著缩短(P<0.05),血清中脂联素含量明显升高(P<0.01),脂肪组织中脂联素mRNA表达明显增多(P<0.05),脂肪组织中脂联素受体表达明显升高(P<0.01),。各治疗组之间差异无统计学意义(P>0.05)。结论:艾灸“神阙”“足三里”穴或两穴合用都能提高AD大鼠血清及脂肪组织中脂联素及脂联素受体水平,进而改善AD的学习记忆能力。Objective To observe the effect of moxibustion on serum adiponectin content,and expression of adiponectin and adiponectin receptor in adipose tissue in Alzheimer’s disease(AD)rats,so as to explore its mechanism underlying improvement of AD.Methods Fifty male SD rats were randomly divided into control,model,Shenque(CV8),Zusanli(ST36)and CV8+ST36 groups(n=10 in each group).The AD model was established by intraperitoneal injection of D-galactose(400 mg·kg-1·d-1)for 5 weeks and scopolamine hydrobromide(3 mg·kg-1·d-1)for 2 weeks.Moxibustion was applied to CV8,ST36 and CV8+ST36 respectively for 3 moxa-cones every time,once daily for 5 weeks.Morris water maze tests were used to assess the rats’ learning-memory ability.The contents of serum adiponectin were assayed using ELISA,and the expression of adiponectin and adiponectin receptor in the adipose tissue was detected by quantitative real-time PCR and Western blot,separately.Results Following modeling,the average escape latency of Morris water maze tests was significantly prolonged(P<0.05),the content of serum adiponectin and the expression level of adiponectin mRNA in adipose tissue were significantly decreased(P<0.01),the expression of adiponectin receptor protein significantly decreased in the model group relevant to the control group(P<0.01).After the intervention,the average escape latency was significantly shortened(P<0.05),the decreased serum adiponectin content and adiponectin mRNA expression,and the decreased adiponectin receptor protein expression in adipose tissue were all reversed in the 3 treatment groups(P<0.01,P<0.05).No significant differences were found among the three moxibustion groups in the above indexes(P>0.05).Conclusion Moxibustion at CV8,ST36 and CV8+ST36 is effective in up-regulating serum adiponectin content,adiponectin mRNA expression and adiponectin receptor protein expression in adipose tissue,which may provide evidence for clinical election of acupoints.

关 键 词：阿尔茨海默病 艾灸 脂肪组织 脂联素 脂联素受体 

分 类 号：R245.81[医药卫生—针灸推拿学]