大鼠创伤性颅脑损伤后不同时间点施加极低频电磁场的神经保护作用  

Protective effect of extremely low frequency magnetic fields irradiation on rats at different time points after traumatic brain injury

作　　者：杨扬[1,2] 程璐璐 李力 刘卫平 Yang Yang;Cheng Lulu;Li Li;Liu Weiping(Key Laboratory for Medical Imaging of Neurological Diseases,Henan Province People's Hospital,Zhengzhou 450003,China;Department of Neurosurgery,Zhumadian City Center Hospital,Zhumadian 463000,China;Department of Neurosurgery,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]河南省人民医院神经疾病影像诊断与研究重点实验室,郑州450003 [2]驻马店市中心医院神经外科,463000 [3]空军军医大学附属西京医院神经外科,西安710032

出　　处：《中华神经医学杂志》2019年第6期575-582,共8页Chinese Journal of Neuromedicine

基　　金：河南省科技攻关计划项目(182102310536).

摘　　要：目的观察极低频电磁场照射对创伤性颅脑损伤(TBI)大鼠的影响。方法450只SD大鼠按随机数字表法分为空白对照组、TBI组、电磁场早期照射组、电磁场晚期照射组、电磁场早期晚期联合照射组,每组90只。后4组大鼠应用连接侧向液压打击(LFP)设备制作TBI模型;空白对照组大鼠施行同样操作,但不连接LFP设备。电磁场早期照射组、电磁场晚期照射组大鼠分别于TBI后6h、60d接受电磁场照射,连续15d;电磁场早期晚期联合照射组于TBI后6h、60d接受2次连续15d的电磁场照射。在TBI后4h、15d和75d,采用免疫组化染色检测各组大鼠海马低氧诱导因子-1α(HIF-1α)的表达;TUNEL染色检测各组大鼠海马区细胞凋亡;检测大鼠脑组织伊文氏蓝(EB)含量和脑水含量;采用水迷宫实验检测大鼠的学习和记忆能力;采用Westernblotting检测大鼠海马组织HIF-1α蛋白的表达。结果TBI后4h、15d和75d,与空白对照组比较,TBI组、电磁场早期照射组、电磁场晚期照射组、电磁场早期晚期联合照射组大鼠海马区凋亡细胞数、HIF-1α蛋白表达量、登上逃生平台的时间差异均有统计学意义(P<0.05)。TBI后15d,电磁场早期照射组、电磁场早期晚期联合照射组大鼠海马区凋亡细胞数、HIF-1α蛋白表达量、登上逃生平台的时间、EB含量和脑水含量低于TBI组,差异均有统计学意义(P<0.05);TBI后75d,电磁场早期照射组、电磁场晚期照射组、电磁场早期晚期联合照射组大鼠海马区凋亡细胞数、HIF-1α蛋白表达量、登上逃生平台的时间低于TBI组,差异均有统计学意义(P<0.05)。结论在TBI后早期和晚期均推荐行极低频电磁场照射,而且照射时间应尽可能延长,有助于神经功能的恢复。Objective To observe the influences of extremely low frequency magnetic fields irradiation in rats at different time points after traumatic brain injury (TBI). Methods According to the random number table method, 450 SD rats were divided into blank control group, TBI group, electromagnetic field early irradiation group, electromagnetic field late irradiation group, and electromagnetic field early and late combined irradiation group (n=90). TBI models in rats of the later four groups were induced by connection of lateral hydraulic impact (LFP) equipment;the rats in the blank control group accepted the same operation, but did not connect to the LFP equipment. The rats in the electromagnetic field early irradiation group and electromagnetic field late irradiation group received electromagnetic field irradiation on 6 and 60 d after TBI for 15 consecutive d, respectively;and the rats in the electromagnetic field early and late combined irradiation group received twice consecutive electromagnetic field irradiation for 15 d on 6 and 60 d after TBI. Four h, 15 d and 75 d after TBI, the hypoxia inducible factor-1α(HIF-1α) expression in the hippocampus of rats in each group was detected by immunohistochemical staining;TUNEL was used to detect cell apoptosis in hippocampal area of rats in each group;the contents of Evans blue (EB) and brain water in rat brain tissues were determined;the water maze experiment was used to test the learning and memory abilities of rats;HIF-1α protein expression in the hippocampal tissues of rats was detected by Western blotting. Results As compared with rats in the blank control group, rats in the TBI group, electromagnetic field early irradiation group, electromagnetic field late irradiation group, and electromagnetic field early and late combined irradiation group had significantly larger number of apoptotic cells in the hippocampus, statistically longer time boarding the escape platform, significantly higher HIF-1α protein expression at 4 h, 15 d and 75 d after TBI (P<0.05). As compared

关 键 词：极低频电磁场 创伤性颅脑损伤 治疗 

分 类 号：R651.15[医药卫生—外科学] R-332[医药卫生—临床医学]