鸡源粪肠球菌对雏鸡致病力试验和毒力基因gelE及efaA的检测  

Pathogenicity Test of Enterococcus faecalis in Chicken and Detection of Virulence Genes gelE and efaA

作　　者：羊玉凤 徐睿[1,2] 陶薪燕 Yang Yufeng;Xu Rui;Tao Xinyan(Department of Animal Science,Xichang University,Sichuan Xichang 615015;Key Laboratory of Prevention ,Control and Detection of Animal Diseases in Panxi,Sichuan Pranxi 615000)

机构地区：[1]西昌学院动物科学学院,四川西昌615015 [2]攀西动物疫病防控与检测四川省高校重点实验室,四川攀西615000

出　　处：《现代畜牧兽医》2019年第5期7-13,共7页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：四川省凉山州农业科技创新项目(15NYCX0040);四川省科技厅支撑计划项目(13ZC1796);四川省教育厅攀西动物疫病防控与检测高校重点实验室(纵341B)

摘　　要：为了解凉山地区鸡源粪肠球菌毒力基因gelE和efaA的基因表达情况,对毒力基因进行检测和致病力试验。通过实验室提供的粪肠球菌10株,进行致病力试验,结果显示,雏鸡死亡率为0～100%,存在较大差异;进行毒力基因gelE(432 bp)、efaA(591 bp)的检测,10株粪肠球菌阳性率均为100%。根据NCBI的基因序列进行粪肠球菌毒力基因gelE、efaA的同源性比对结果均为100%。In order to understand the gene expression of virulence genes gelE and efaA of Enterococcus faecalis in chicken in Liangshan area, virulence genes were detected and virulence tests were carried out. Ten strains of Enterococcus faecalis provided by the laboratory were tested for pathogenicity. The results showed that the mortality rate of chickens was 0~100% and there were significant differences. The positive rates of 10 strains of Enterococcus faecalis were all 100% by detecting the virulence genes gelE(432 bp) and efaA(591 bp). According to NCBI gene sequence, the results of homology comparison of virulence genes gelE and efaA of Enterococcus faecalis were both 100%.

关 键 词：鸡源 粪肠球菌 毒力基因 致病力试验 

分 类 号：S852.611[农业科学—基础兽医学]