VEGF和Bcl-2双基因修饰的MSC对缺氧心肌细胞凋亡的影响  

Bcl-2 and VEGF dual genetic co-overexpressing mesenchymal stem cells protect cardiomyocytes under hypoxia in vitro via paracrine effect

作　　者：倪晓彬[1] 蔡志雄[1] 李和慨 易敏[2] NI Xiao - bin;CAI Zhi - xiong;LI He - kai;YI Min(Department of Cardiology, Shantou central hospital,Shantou 515031,Guangdong,China)

机构地区：[1]汕头市中心医院心血管内科,广东汕头515031 [2]南方医科大学珠江医院心脏中心,广东广州510280

出　　处：《广东医学》2019年第11期1542-1545,共4页Guangdong Medical Journal

摘　　要：目的探讨VEGF和Bcl-2双基因修饰的骨髓间充质干细胞(MSC)对缺氧心肌细胞的作用及机制。方法原代培养新生Sprague-Dawley大鼠心肌细胞,使用Transwell共培养系统将心肌细胞与MSC或MSC+BV置于1%O224h缺氧处理。实验分组:正常培养组、缺氧培养组、与MSC共培养缺氧组、与MSC+BV共培养缺氧组。采用TUNEL染色法、AnnexinV-FITC/PI流式细胞术分析心肌细胞凋亡。结果TUNEL染色及流式细胞术结果均表明缺氧增加了心肌细胞凋亡,与MSC共培养缺氧组心肌细胞较对照组凋亡减少(P<0.05),而与MSC+BV共培养缺氧组心肌细胞对比与MSC共培养缺氧组凋亡更少,差异有统计学意义(P<0.01)。结论VEGF和Bcl-2双基因修饰的MSC通过旁分泌效应减少缺氧心肌细胞凋亡。Objective To investigate the protective effect of Bcl - 2 and VEGF dual genetic co - overexpressing mesenchymal stem cells ( MSCs) on cardiomyocytes under anoxic in vitro and its mechanism.Methods Neonatal SD rat cardiomyocytes were cultured in vitro.Cardiomyocytes were co - cultured with either MSC - GFP or MSC - BV using Tran- swell coculture system.The condition of 94% N2,5% CO2,1% O2,for 24 h was used. Cells were divided into four groups,normal culture group,hypoxia culture group,coculture with MSC - GFP group,and coculture with MSC + BV group.Flow cytometric assay and TUNEL assay were used to analyzed the apoptosis of cardiomyocytes.Results Flow cy- tometric assay and TUNEL assay showed that the apoptosis of cardiomyocytes that cocultured with Bcl - 2 and VEGF dual genetic modification MSC under hypoxia condition was significantly reduced.Conclusion Bcl - 2 and VEGF dual genetic co - overexpressing MSCs protect cardiomyocytes under hypoxia in vitro via paracrine effect.

关 键 词：BCL-2 VEGF 骨髓间充质干细胞 旁分泌 缺氧 心肌细胞 

分 类 号：R542.2[医药卫生—心血管疾病] R364.4[医药卫生—内科学]