硫酸黏菌素残留对土壤固氮微生物nifH基因多样性的影响  

Effects of colistin sulfate residue on the nifH gene diversity of soil nitrogen-fixing microorganisms

作　　者：范葶莉 彭金菊[1] 孙永学[3] 牛金利 钟晓霞[3] 王勉之[3] 苏恺[1] 马驿[1] Tingli;PENG Jinju;SUN Yongxue;NIU Jinli;ZHONG Xiaoxia;WANG Mianzhi;SU Kai;MA Yi(Agricultural College,Guangdong Ocean University,Zhanjiang Guangdong 524088;Department of Animal Husbandry and Veterinary Medicine,Cangzhou Technical College,Cangzhou Hebei 061001;Guangdong Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation,South China Agricultural University,Guangzhou Guangdong 510642)

机构地区：[1]广东海洋大学农学院,广东湛江524088 [2]沧州职业技术学院畜牧兽医系,河北沧州061001 [3]华南农业大学广东省兽药研制与安全评价重点实验室,广东广州510642

出　　处：《环境污染与防治》2019年第6期663-667,671,共6页Environmental Pollution & Control

基　　金：广东省自然科学基金资助项目(No.2017A030313133);广东省自然科学基金重点资助项目(No.2016A030311029)

摘　　要：为了探讨硫酸黏菌素残留对土壤固氮微生物nifH基因多样性的影响,在土壤中添加0、0.5、5.0、50.0mg/kg质量浓度的硫酸黏菌素,分别于7、21、35、49d时采集土壤样品,提取土壤总DNA,采用末端限制性片段长度多样性分析技术对土壤固氮微生物nifH基因进行多样性分析。结果表明,添加硫酸黏菌素处理的nifH基因分类操作单元数均比不添加硫酸黏菌素处理的少。硫酸黏菌素影响土壤固氮微生物nifH基因的多样性,使土壤固氮微生物的丰富度和均匀度降低。主成分分析发现,21d时,硫酸黏菌素对土壤微生物群落结构改变的影响最大,随着时间的延长,硫酸黏菌素对土壤固氮微生物群落结构多样性的影响有所减弱。The effects of colistin sulfate on the nifH gene diversity of soil nitrogen-fixing microorganisms were investigated.Colistin sulfate was added in the soil with mass concentrations of 0,0.5,5.0 and 50.0 mg/kg.Soil samples were collected at 7,21,35 and 49 d,respectively.Total DNA was extracted and the diversity of nifH genes was analyzed by terminal restriction fragment length polymorphism.Results showed that operational taxonomic units for nifH gene of colistin sulfate added treatments were lower than that of colistin sulfate not added treatment.Colistin sulfate affected the nifH gene diversity,making it abundance and homogeneity decreasing.Principal component analysis showed that colistin sulfate had the most significant effects on soil microbial community structure at 21 d.With time going on,the effects weakend.

关 键 词：硫酸黏菌素 固氮微生物 基因多样性 土壤 

分 类 号：X53[环境科学与工程—环境工程] S154.3[农业科学—土壤学]