人参皂苷Rg2对慢性坐骨神经损伤大鼠痛觉敏化和抑郁状态的影响  

Effects of ginsenoside-Rg2 on mechanical allodynia,heat hyperalgeia,depressive state of rats with chronic sciatic nerve constriction injury

作　　者：张巧莲 李双英 李平 ZHANG Qiao-lian;LI Shuang-ying;LI PING(Department of Neurology,Characteristic Medical Center of the Chinese People’s Armed Police Forces,Tianjin 300162,China)

机构地区：[1]中国人民武装警察部队特色医学中心神经内科

出　　处：《中国应用生理学杂志》2019年第3期228-231,288,共5页Chinese Journal of Applied Physiology

摘　　要：目的:观察人参皂苷Rg2对慢性坐骨神经损伤大鼠痛觉敏化、抑郁状态的影响。方法:将50只SD大鼠随机分为5组(n=10):空白对照组(Normal+生理盐水腹腔注射)、假手术组(手术但不结扎+生理盐水腹腔注射)、坐骨神经慢性压迫损伤(CCI)组(CCI+生理盐水腹腔注射)、人参皂苷Rg2低剂量组(CCI+Rg25mg/kg腹腔注射)、人参皂苷Rg2高剂量组(CCI+Rg210mg/kg腹腔注射)。CCI模型建立后,药物通过注射器进行腹腔内注射5ml/kg,每天1次,连续14d。分别在术前1d和术后1、3、5、7、10、14d测定大鼠的机械性缩足反射阈值(MWT)和热缩足潜伏期(TWL);术前1d和术后第14日时检测明暗箱实验和强迫游泳试验。结果:与假手术组比较,CCI组术后14d机械痛阈值和热痛潜伏期明显缩短(P<0.01),明箱内停留时间明显缩短(P<0.01),穿梭次数明显减少(P<0.01),游泳潜伏期明显延长(P<0.01)。与CCI组比较,人参皂苷Rg2组术后14d机械痛阈和热痛潜伏期明显增加(P<0.01),大鼠在明箱内时间明显延长(P<0.01),穿梭次数明显增多(P<0.01),且游泳潜伏期明显缩短(P<0.01)。结论:人参皂苷Rg2能抑制CCI大鼠的机械痛敏和热痛敏,同时改善其抑郁状态。Objective: To investigate the effects of ginsenoside-Rg2 on mechanical allodynia,heat hyperalgeia,depressive state of rats with chronic sciatic nerve constriction injury. Methods: Fifty SD rats were randomly divided into 5 groups: blank control group( Normal,normal + saline),sham operation group( Sham,sham operation + saline),chronic constriction injury of the sciatic nerve group( CCI,CCI + saline),ginsenoside-Rg2 low dose group( CCI + Rg25 mg/kg),and ginsenoside-Rg2 high dose group( CCI + Rg210 mg/kg).After the CCI model was established,drug were injected into the abdominal cavity through the syringe once a day,for 14 consecutive days.The mechanical shrinkage foot reflex threshold( MWT) and thermal withdrawal latency( TWL) were determined at 1 d before the operation and at 1,3,5,7,10 and 14 d after the operation.Light-dark transition test,forced swimming test were determined at1 d before the operation and at 14 d after the operation.Results: Compared with the sham group,the MWL and TWL of the CCI rats were decreased significantly( P<0.01),time in the light compartment and number of transition were decreased( P<0.01),the immobility time in FST was also prolonged significantly( P<0.01). At 14 days after CCI operation,the MWL and TWL of the ginsenosideRg2 groups were increased significantly( P<0.01),time in the light compartment and number of transition were also shortened significantly( P<0.01),the immobility time in FST was also shortened significantly( P<0.01). Conclusion: Intraperitoneal injection of ginsenoside-Rg2 can inhibit the mechanical and thermal pain sensitivity of CCI rats,and can relieve depressive state.

关 键 词：人参皂苷RG2 慢性坐骨神经损伤 机械痛敏 热痛敏 抑郁状态 大鼠 

分 类 号：R651.3[医药卫生—外科学]