大豆根际溶磷真菌的筛选、复配及包埋固定化回用效果  

Screening, Compounding and Embedding Immobilization Effect of Soybean Rhizosphere Phosphate Solubilizing Fungi

作　　者：曾齐 王继华[1] 隋海潮 车琦 张必弦[2] 刘秀林[2] Zeng Qi;Wang Jihua;Sui Haichao;Che Qi;Zhang Bixian;Liu Xiulin(School of Life Science and Technology, Harbin Normal University, Harbin, 150025;Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, 150025)

机构地区：[1]哈尔滨师范大学,生命科学与技术学院,哈尔滨150025 [2]黑龙江省农业科学院,大豆研究所,哈尔滨150086

出　　处：《分子植物育种》2019年第10期3353-3363,共11页Molecular Plant Breeding

基　　金：国家重大专项项目“水体污染控制与治理”(2017ZX07202-002-06)资助

摘　　要：为获得具高效溶磷能力的溶磷真菌,本研究从大豆根际土壤中分离筛选出5株具有溶解Ca3(PO4)2、AlPO4、FePO4及磷矿粉能力的高效菌株,分别命名为Z2、Z3、Z8、T4和Y2,分析了其对不同磷源的溶解能力,复配了最优溶磷组合同时评价了最优组合固定化回用的效果。结果表明:菌株Z2为黄色蓝状菌(Talaromyces flavus),Z3为绳状篮状菌(Talaromyces funiculosus),Z8为黑曲霉(Aspergilus niger),T4为嗜松蓝状菌(Talaromyces pinophilus),Y2为糙刺蓝状菌(Talaromyces trachyspermus)。培养11 d内,Z2、Z3、Z8、T4及Y2对Ca3(PO4)2的最大溶磷量分别为610.51 mg/mL、674.39 mg/mL、687.10 mg/mL、667.06 mg/mL和629.08 mg/m L,对Al PO4的最大溶磷量分别为485.76 mg/mL、505.59 mg/mL、559.72 mg/m L、495.97 mg/mL和540.02 mg/mL,对FePO4的最大溶磷量分别为504.60 mg/mL、511.57 mg/m L、553.21 mg/mL、508.23 mg/m L和520.42 mg/mL以及对磷矿粉的最大溶磷量分别为139.78mg/mL、135.31mg/mL、159.03mg/mL、154.61mg/m L和154.43mg/mL。溶磷过程中,溶磷量与pH值呈现极显著的负相关性,并测得5株真菌释放有机酸含量分别为228.6 mL/L、91.5 mL/L、7 254.1 mL/L、314.4 mL/L和284.6 mL/L。对无拮抗反应的菌株进行随机复配组合,发现菌株Z3、Z8和Y2组成的复配组合溶磷能力最强,其对Ca3(PO4)2溶磷量高达779.94 mg/m L。最优复配组合包埋固定回用至土壤后,土壤中有效磷含量得到明显提升,最高效磷含量可增长至113.5 mg/kg,增长率为127.3%。本研究为微生物溶磷菌肥的开发提供菌种资源,也可为今后的进一步应用研究提供科学依据。In order to obtain phosphorus-dissolving fungi with high-efficiency phosphorus-dissolving ability,five high-efficiency strains with the ability to dissolve Ca3(PO4)2,AlPO4,FePO4and phosphate rock powder were isolated and isolated from soybean rhizosphere soil,respectively named Z2.Z3,Z8,T4 and Y2,the solvency of different phosphorus sources was analyzed,and the optimal phosphorus combination was compounded.The effect of optimal combination and reuse was evaluated.The results showed that the strain Z2 was Talaromyces flavus,Z3was Talaromyces funiculosus,Z8 was Aspergilus niger,T4 was Talaromyces pinophilus,and Y2 was rough.Talaromyces trachyspermus,the maximum phosphorus uptake of Ca3(PO4)2by Z2,Z3,Z8,T4 and Y2 was 610.51 mg/m L,674.39 mg/m L,687.10 mg/mL,667.06 mg/mL and 629.08 mg/mL within 11 days of culture,respectively.The maximum phosphorus solubilization of Al PO4was 485.76 mg/mL,505.59 mg/mL,559.72 mg/m L,495.97 mg/m L and 540.02 mg/m L,respectively.The maximum phosphorus solubilization of FePO4was 504.60 mg/m L,511.57 mg/mL,553.21 mg/mL,508.23 mg/mL and 520.42 mg/mL and phosphate rock.The maximum phosphorus solubilization of the powder was 139.78 mg/mL,135.31 mg/m L,159.03 mg/mL,154.61 mg/m L and 154.43 mg/mL respectively.In the process of phosphorus solubilization,the amount of dissolved phosphorus showed a significant negative correlation with the pH value.The organic acid content of the five fungi was 228.6 m L/L,91.5 m L/L,7 254.1 m L/L,314.4 mL/L and 284.6 mL/L.Randomly compounded the strains without antagonistic reaction,it was found that the combination of strains Z3,Z8 and Y2 had the strongest ability to dissolve phosphorus,and the amount of phosphorus dissolved in Ca3(PO4)2was as high as 779.94 mg/mL.After the optimal compounding combination was embedded and fixed to the soil,the available phosphorus content in the soil was significantly improved,and the most efficient phosphorus content could be increased to 113.5 mg/kg,with a growth rate of 127.3%.This study provides strain resources for the dev

关 键 词：溶磷真菌 筛选 复配组合 包埋固定化 

分 类 号：S154.3[农业科学—土壤学] S565.1[农业科学—农业基础科学]