工业大麻雄性相关RAPD和SCAR标记的筛选与鉴定  

Screening and Identification of Male-Specific RAPD and SCAR Markers in Cannabis sativa L.(Industrial Hemp)

作　　者：姜颖[1,2] 冯乃杰 王晓楠[1] 韩喜财[1] 韩承伟[1] 赵越[1] 曹焜[1] 孙宇峰 李振伟[1] Jiang Ying;Feng Naijie;Wang Xiaonan;Han Xicai;Han Chengwei;Zhao Yue;Cao Kun;Sun Yufeng;Li Zhenwei(Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319, Heilongjiang, China;College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China)

机构地区：[1]黑龙江省科学院大庆分院,黑龙江大庆163319 [2]黑龙江八一农垦大学农学院,黑龙江大庆163319

出　　处：《作物杂志》2019年第3期66-72,共7页Crops

基　　金：黑龙江省科学院院长基金(YZ2018DQ02);黑龙江省青年科学基金(QC2016037)

摘　　要：利用42条RAPD(Random Amplified Polymorphic DNA)随机扩增引物分析工业大麻品种"火麻一号"组成的雄性或雌性DNA池(DNA pools),结果显示,引物OPV-08扩增得到一条大小为869bp与工业大麻雄性相关的特异条带。根据测序结果,合成了两条SCAR(Sequence Characterized Amplified Region)标记引物,该SCAR标记不仅可以对工业大麻雌雄异株材料花期已知性别的雌雄植株进行准确鉴定,还可以对幼苗期未知性别的大麻雌雄植株进行鉴定;也可对雌雄同株材料可能出现的雄化进行早期鉴定。这不仅为工业大麻早期性别鉴定提供基础,且为减少雌雄同株材料的雄化提供支撑。The male DNA pools or female DNA pools of "Huoma No.1" were analyzed respectively by 42 RAPD random amplified primers.The results showed that a male-specific DNA fragment with a length of 869 bp was amplified by OPV-08.Two SCAR primers were constructed according to the sequencing results.The primers of SCAR marker could identify the different genders of hemp not only in the known sex plants at flowering stage but also in the unknown sex seedlings at seedling stage.That could also be used for early identification of the appearance of male plant in monoecious industrial hemp.This provides a basis for early sex identification of industrial hemp,also provides support for reducing the appearance of male plant in monoecious industrial hemp.

关 键 词：工业大麻 性别鉴定 雄性 RAPD SCAR 

分 类 号：S563.3[农业科学—作物学]