利用SNP芯片解析油菜杂交种丰油10号的遗传基础  

Genetic Analysis of Rape Hybrid Fengyou No.10 Using SNP Chips

作　　者：蔡东芳[1] 张书芬[1] 王建平[1] 曹金华[1] 文雁成[1] 张书法 何俊平[1] 赵磊[1] 王东国[1] 朱家成[1] Cai Dongfang;Zhang Shufen;Wang Jianping;Cao Jinhua;Wen Yancheng;Zhang Shufa;He Junping;Zhao Lei;Wang Dongguo;Zhu Jiacheng(Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture and Rural Affairs/Key Laboratory of Oil Crop Genetic Improvement in Henan Province, Zhengzhou 450002, Henan, China;Tanghe Seed Technical Service Station of Henan Province, Tanghe 473400, Henan, China)

机构地区：[1]河南省农业科学院经济作物研究所/农业农村部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室,河南郑州450002 [2]河南省唐河县种子技术服务站,河南唐河473400

出　　处：《作物杂志》2019年第3期80-85,共6页Crops

基　　金：十三五国家重点研发计划(2018YFD0100600);国家现代农业产业技术体系-油菜(CARS-12);河南省重大科技专项(151100111200);河南省农业科学院优秀青年科技基金(2018YQ06)

摘　　要：为解析高产稳产油菜品种丰油10号的分子遗传学基础,利用油菜60K SNP芯片对丰油10号及包括其2个亲本在内的20份骨干亲本系进行了基因型分析。结果表明:20份骨干亲本系间的遗传距离变幅为0.123~0.463,平均为0.348。丰油10号母本22A和父本P087-2间遗传距离为0.375,高于平均遗传距离。聚类分析将20份骨干亲本系划分为4个类群,其中母本22A归在类群Ⅰ,父本P087-2归在类群Ⅳ。基因组比较发现杂交种丰油10号与母本22A的相似性比例为64.77%,与父本P087-2的相似性比例为62.89%,其中有30.78%的基因组杂合区段来源于双亲不同基因型间的组合。同时发现丰油10号与父母本在19条染色体上的多态性比例存在比较大的差异。该研究为后续油菜品种的选育提供理论指导。In order to analyze the molecular genetic basis of rape hybrid Fengyou No.10,Fengyou No.10 and 20 elite inbred lines including its parents were genotyped using the Illumina 60 K SNP chip technology.The results showed that the genetic distance between pairs of 20 elite inbred lines ranged from 0.123 to 0.463,with an average of 0.348.The genetic distance between the female parent 22 A and male parent P087-2 of Fengyou No.10 was 0.375,which was higher than the average value of 20 elite inbred lines.All these inbred lines were divided into four groups using cluster analysis,in which the female parent 22 A and male parent P087-2 belong to the first group and the fourth group,respectively.Comparative genome analysis found that the hybrid Fengyou No.10 had the similarity of 64.77% and62.89% with its female parent 22 A and male parent P087-2,respectively.The 30.78% loci of Fengyou No.10 were heterozygous.At the same time,the great differences of the polymorphism rate in 19 chromosomes were observed between Fengyou No.10 and the parents.Our study would provide a theoretical guide for subsequent variety breeding in Brassica napus.

关 键 词：甘蓝型油菜 SNP芯片 遗传距离 遗传基础 

分 类 号：S565.4[农业科学—作物学]