二氧化碳对小鼠肺脏及气管壁中IL-6和TNF-α表达的影响  

Effects of carbon dioxide on the expression of IL-6 and TNF-α in lung and tracheal wall of mice

作　　者：张艳英[1] 崔嘉[2] 陈宝江[2] 崔亚利[1] 胡满[1] ZHANG Yanying;CUI Jia;CHEN Baojiang;CUI Yali;HU Man(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071000,China;College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学动物医学院,河北保定071000 [2]河北农业大学动物科技学院,河北保定071000

出　　处：《黑龙江畜牧兽医》2019年第11期17-19,24,179-180,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家重点研发计划项目(2016YFD0500501)

摘　　要：为了探讨二氧化碳对动物呼吸系统影响的机制,试验选取40只30日龄健康状态良好的KM小鼠随机分成4组,每组10只,第1组小鼠在正常环境中饲养,第2~4组小鼠饲养环境中二氧化碳浓度分别是1500,2500,3 500mg/m^3,试验周期为14d。采用免疫组织化学染色和图像分析技术对肺脏和气管壁黏膜中白细胞介素-6(IL-6)、肿瘤坏死因子(TNF-α)的表达情况进行观察与分析。结果表明:IL-6免疫反应阳性物质在呼吸性终末细支气管的黏膜层、肺泡上皮细胞、气管壁黏膜层分布最多,TNF-α在肺泡隔、肺泡Ⅱ型上皮细胞分布最多,其平均灰度值均为第4组最高且显著或极显著高于第1组(P<0.05或P<0.01)。说明随着环境中二氧化碳浓度的升高,小鼠肺脏和气管壁中IL-6和TNF-α的表达量增多,高浓度二氧化碳会使肺脏和气管壁黏膜发生炎症反应。In this study, forty 30-day-old KM mice were randomly divided into four groups with ten in each group. The mice in group 1 were raised in normal environment;In group 2 to 4, animals were fed at a concentration of 1 500, 2 500,3 500 mg/m^3 of carbon dioxide, respectively. The trial period was 14 d. The expression of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in lung and tracheal wall were observed and analyzed by immunohistochemical staining and image analysis methods.The results showed that IL-6 immunoreactive positive materials were mainly distributed in the mucous layer of the pulmonary terminal bronchioles, alveolar epithelium cells, mucous layer of tracheal wall. The TNF-α immunoreactive positive materials were distributed in the alveolar septum and type Ⅱ alveolar cells. Moreover, the average gray value of immunoreactive positive materials in lung and trachea wall of mice was the highest in group 4 and higher than group 1 ( P<0. 05 or P<0. 01 ). It suggested that the expression of IL-6 and TNF-α in the lung and tracheal wall of mice increase with increasing of carbon dioxide concentration. High concentrations of carbon dioxide can cause an inflammatory reaction in the lung and tracheal wall mucosa.

关 键 词：二氧化碳 小鼠 肺脏 气管壁 白细胞介素-6 肿瘤坏死因子-α 

分 类 号：S865.1[农业科学—野生动物驯养]