绿侧花海葵寡肽对CCl4引起的小鼠急性肝损伤模型的影响  

Effect of Anthopleura anjunae Peptide on Acute Liver Injury in Mice Induced by CCl4

作　　者：王予菲 唐云平 李小娟 刘成娟 陈亚南 杲亚许 杨最素 丁国芳 WANG Yu-fei;TANG Yun-ping;LI Xiao-juan(School of Food and Medicine of Zhejiang Ocean University, Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, Zhoushan 316022;Marine Fisheries Research Institute of Zhejiang Province, Zhoushan 316021, China)

机构地区：[1]浙江海洋大学食品与医药学院,浙江省海洋生物医用制品工程技术研究中心,浙江舟山316022 [2]浙江省海洋水产研究所,浙江舟山316021

出　　处：《浙江海洋大学学报（自然科学版）》2019年第2期127-133,共7页Journal of Zhejiang Ocean University：Natural Science

基　　金：国家自然科学基金(81773629);国家海洋重大计划项目(201586-2);浙江省科技厅重大专项(2013C03036);浙江省自然科学基金(LS15H30001)

摘　　要：研究绿侧花海葵寡肽(APP-H)对四氯化碳(CCl4)引起的小鼠急性肝损伤模型的影响。48只ICR小鼠随机分成6组,每组8只:正常对照组、肝损伤模型组、绿侧花海葵寡肽AAP-H低剂量组(75mg·kg^-1)、AAP-H中剂量组(150mg·kg^-1)、AAP-H高剂量组(300mg·kg^-1)和阳性对照组(联苯双酯(150mg·kg^-1))。连续灌胃7天,正常组与模型组用等计量纯水灌胃。第7天末采用腹腔注射1%CCl4橄榄油溶液制备小鼠急性肝损伤模型,测定小鼠血清ALT、AST和小鼠肝脏组织匀浆中GSH-Px、SOD、MDA水平,HE染色观察APP-H对小鼠肝脏的病理学影响及微观结构影响。结果表明,AAP-H可降低小鼠血清中ALT、AST活性(P<0.05),抑制肝组织中MDA的上升(P<0.05),上调SOD和GSH-Px活性(P<0.05),使肝细胞的损伤减轻。本实验初步证明APP-H对CCl4致小鼠急性肝损伤有一定的保护作用。Our studies supposed to investigate the effect of Anthopleura anjunae Peptide on the acute liver injury in mice that induced by CCl4. ICR mice (48) were randomly divided into 6 groups (each group contains eight ICR mice): normal control group, model group, A. anjunae treated groups (75 mg·kg^-1, 150 mg·kg^-1 and 300 mg·kg^-1, respectively) and positive drug group (150 mg·kg^-1 of bifendate). The A. anjunae or bifendate treated groups were given the same amount of drugs for seven days, and the control and model groups were given the equal volume of water. Two hours after the last administration, mice were given intraperitoneal injection with 1% CCl4 to make the acute liver injury model. Then, the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the serum of each mice were detected. The concentration of hepatic malondialdehyde (MDA) was also determined in the present study. In addition, the changes of hepatic pathological were observed by using HE staining and transmission electron microscope. Our results showed that AAP-H has an effect on reducing the activities of serum ALT and AST (P<0.05), inhibiting the level of MDA (P<0.05) and up-regulating the activities of SOD and GSH-Px (P<0.05). It was preliminarily indicated that AAP-H has certain protection against the acute liver injury induced by CCl4 in mice.

关 键 词：绿侧花海葵寡肽 急性肝损伤 四氯化碳 护肝 

分 类 号：R282.77[医药卫生—中药学]