日本红枫‘青龙’组织培养与快速繁殖  

Tissue culture and rapid propagation of the Acer palmatum’seiryu’

作　　者：孙红英[1,2] 辛全伟[1] 林兴生[2] 罗海凌[2] 林辉[2] 马志慧[1] 严少娟 兰思仁[1] SUN Hongyingb;XIN Quanwei;LIN Xingsheng;LUO Hailing;LIN Hui;MA Zhihui;YAN Shaojuan;LAN Siren(School of Forestry,Fujian China Fuzhou 350002;National Engineering Research Center of Juncao,Fujian China Fuzhou 350002;School of Resources and Environment,Fujian Agriculture and Forestry University,Fujian China Fuzhou 350002)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学国家菌草工程技术研究中心,福建福州350002 [3]福建农林大学资源与环境学院,福建福州350002

出　　处：《中南林业科技大学学报》2019年第7期44-47,共4页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(31500265);福建农林大学第三批科技创新专项基金项目

摘　　要：以青龙半木质化枝条为外植体,采用丛生芽诱导途径,建立起组培快繁体系。结果表明:最佳启动培养基为WPM+0.05mg/LTDZ+30g/L蔗糖+6.5g/L琼脂,启动率为96.1%;最佳增殖培养基为WPM+0.06mg/LTDZ+0.3mg/LIBA+6.5g/L琼脂+30g/L蔗糖,继代周期30d,增殖系数高达4.8;最佳生根培养基为1/2WPM+0.3mg/LNAA+6.5g/L琼脂+30g/L蔗糖,培养30d,其生根率达96.7%。炼苗后,移栽到珍珠岩∶蛭石∶泥炭土体积比为1∶1∶1的混合基质中,成活率达到90%以上。The explants were taken from the late and half lignificational stems with shoot in the adult selected trees. An economic and high effective technology system for rapid propagation of Acer palmatum ‘seiryu’was established, through multiple-shoots proliferation. The result show that the optimal starting medium was modified WPM + 0.05 mg/L TDZ+ 30 g/L sucrose + 6.5 g/L gelatin, the establishment rates were 96.1%;the optimal mulitiplication medium was modified WPM +0.06 mg/L TDZ+0.3 mg/L IBA + 30 g/L sucrose + 6.5 g/L gelatin, the subculture cycle was 30 d, the highest multiplication rate was 4.8;the optimal rooting medium was 1/2 WPM + 0.3 mg/L NAA + 30 g/L sucrose + 6.5 g/L gelatin, the rooting rate has reached 96.7% within 30 d. The transplanting matrix were pearlite,vermiculite and peat (1 : 1 : 1, V / V / V), and its corresponding transplanting survival rate was over 90%.

关 键 词：日本红枫'青龙’ 组织培养 最佳培养基 快速繁殖 

分 类 号：S723.132[农业科学—林木遗传育种] S687.9[农业科学—林学]