基于iTRAQ技术筛选东乡野生稻根系低温响应蛋白  

Identification of Cold Responsive Proteins in Dongxiang Wild Rice Root Based on iTRAQ Technique

作　　者：唐秀英 王会民 龙起樟 黄永兰 芦明 曹志斌 万建林 Tang Xiuying;Wang Huimin;Long Qizhang;Huang Yonglan;Lu Ming;Cao Zhibin;Wan Jianlin(Jiangxi Super-rice Research and Development Center,Nanchang,330200)

机构地区：[1]江西省超级水稻研究发展中心

出　　处：《分子植物育种》2019年第11期3624-3631,共8页Molecular Plant Breeding

基　　金：江西省农业科学院青年基金项目(2015CQN001);江西省科技计划重大项目(20143ACF60008)共同资助

摘　　要：为揭示东乡野生稻的耐冷机理,本研究以东乡野生稻为材料,分别进行6℃(低温)和25℃(对照)处理3 d,采用iTRAQ技术开展东乡野生稻苗期根系的差异蛋白质组分析。结果表明,利用iTRAQ技术共鉴定到146个差异表达蛋白质(p<0.05且差异倍数>1.2或<0.83),其中,上调表达48个,下调表达98个。GO分析表明,差异表达蛋白质主要参与代谢过程、细胞过程、单生物过程和刺激反应等,涉及到细胞、细胞成分、细胞器、细胞器成分和膜等细胞组分,主要发挥结合和催化活性功能。KEGG富集分析显示,差异表达蛋白质主要参与代谢途径和次级代谢产物的生物合成。48个表达上调的蛋白质主要涉及逆境胁迫与信号传导、呼吸作用与能量代谢以及生长发育等功能,可以作为东乡野生稻耐冷性研究的候选蛋白。这些鉴定到的差异蛋白质为进一步阐明东乡野生稻的耐冷机理提供理论依据。In order to reveal the chilling tolerant mechanism of Dongxiang wild rice,using Dongxiang wild rice as material,differential proteome analysis of Dongxiang wild rice roots in seedling stage was carried out based on iTRAQ technique at 6℃(low temperature)and 25℃(control)for 3 d,respectively.The results indicated that a total of 146 differentially expressed proteins(fold change ratio>1.2 or<0.83,p<0.05)were identified using iTRAQ technique,in which 48 were up-regulated and 98 were down-regulated.GO analysis indicated that the differentially expressed proteins mainly participated in the metabolic process,cellular process,single-organism process and response to stimulus,etc..They involved in cell,cell part,organelle,organelle part and membrane and other cell components,mainly serving binding and catalytic activity function.KEGG enrichment analysis showed that differentially expressed proteins mainly participated in metabolic pathways and biosynthesis of secondary metabolites.The 48 up-regulated proteins primarily involved functions of adversity stress,signal transduction,respiration,energy metabolism and growth and development,which could be used as candidate proteins of studies on cold tolerance of Dongxiang wild rice.These identified differentially expressed proteins could provide theoretical foundation for further elucidating the chilling tolerant mechanism of Dongxiang wild rice.

关 键 词：东乡野生稻 低温胁迫 差异表达蛋白 iTRAQ技术 

分 类 号：S511.9[农业科学—作物学]