6份披碱草属牧草种质材料抗寒性的比较  

Comparison of chilling resistance of six Elymus germplasms

作　　者：陈玖红 王沛 王平 胡健[1] 周青平[1] CHEN Jiuhong;WANG Pei;WANG Ping;HU Jian;ZHOU Qingping(College of Life Science & Technology,Southwest Minzu University,Chengdu 610041,Sichuan,China)

机构地区：[1]西南民族大学生命科学与技术学院

出　　处：《草业科学》2019年第6期1591-1599,共9页Pratacultural Science

基　　金：西南民族大学研究生创新型科研项目(CX2018SZ63);青藏高原生态畜牧业协同创新中心开放基金(QZGYXT04)

摘　　要：低温是限制植物生长发育的主要因素之一。本研究以6份披碱草属(Elymus)牧草种质为试验材料,分别对冷胁迫(5 ℃) 8、16和24d后各种质材料幼苗的相对质膜透性、相对含水量、丙二醛含量、叶绿素含量及渗透调节物质的积累和抗氧化酶活性等指标的变化率(低温处理下的测定值/对照下的测定值)进行分析对比,探讨冷胁迫下几种披碱草属牧草抗寒性的差异,旨在为高寒地区的抗寒披碱草种质筛选提供理论依据。结果发现,在低温处理24d的过程中,6份种质材料都表现出不同程度的冷适应性。通过对相对质膜透性变化率、相对含水量变化率、丙二醛含量变化率和叶绿素含量变化率进行隶属函数分析,发现生长在高海拔地区(>2900m)的老芒麦(Elymus sibiricus,编号12-9-1)、圆柱披碱草(E.breviaristatus,编号11-6)、垂穗披碱草(E.nutans,编号09-214)在长期适应寒冷环境的过程中,通过保持冷胁迫下低的相对质膜透性和高的渗透调节能力以维持细胞内水分和叶绿素含量,表现出更强的抗寒能力。Low temperature is one of the important limiting factors affecting plant growth.The chilling resistances of six Elymus germplasms were evaluated,and,after 8,16,and 24 days of chilling treatment (5 ℃),plants were harvested and their relative membrane permeability,relative water content,malondialdehyde (MDA) content,chlorophyll content,accumulation of osmotic regulation substances and antioxidant enzyme activities,and change rates (measured value under low temperature treatment/measured value under control) were analyzed and compared.The aim of this work was to explore the differences in cold resistance among the plant materials used,to provide a theoretical basis for the selection of Elymus lanceolata germplasm in alpine regions.The results showed that,in the process of low-temperature treatment for 24 days,all the six tested materials showed different degrees of cold adaptability.Through subordinate function analysis of the changes in relative plasmalemma permeability,relative water content,and MDA and chlorophyll content,it was found that 12-9-1,11-6, and 09-214 which grow at high altitudes could maintain their water and chlorophyll content by maintaining low relative membrane permeabilities and high osmotic adjustment abilities under chilling stress to exhibit a stronger chilling resistance.

关 键 词：披碱草属 冷胁迫 隶属函数 抗寒性 

分 类 号：S543.903.4[农业科学—作物学]