响应面法优化黄芪总黄酮提取工艺及初步纯化  

Optimization of extraction technology for total flavonoids from astragali radix by response surface methodology and their preliminary enrichment

作　　者：袁惠君[1] 李欣 贾鸿震 袁毅君[3] YUAN Huijun;LI Xin;JIA Hongzhen;YUAN Yijun(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,Gansu,China;Gansu Research Institute of Light Industry Co.Ltd,Lanzhou 730000,Gansu,China;Tianshui Normal University,Tianshui 741000,Gansu,China)

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050 [2]甘肃省轻工研究院有限责任公司,甘肃兰州730000 [3]天水师范学院,甘肃天水741000

出　　处：《草业科学》2019年第6期1683-1691,共9页Pratacultural Science

基　　金：国家自然科学基金(81360619)

摘　　要：黄芪总黄酮具有抗氧化、增强机体免疫等功能,常作为免疫增强剂添加在食品和饲料中。通过单因素试验研究乙醇体积分数、料液比和提取时间对黄芪总黄酮提取量的影响,进一步用Box-Behnken法优化黄芪总黄酮最佳提取工艺,并利用大孔吸附树脂-聚酰胺联用技术对其进行富集纯化。结果表明:在乙醇体积分数70%,料液比1∶17,提取时间70min的条件下提取效果最佳,总黄酮提取量为2.78mg·g^-1,相对标准偏差为1.41%。经HPD722大孔吸附树脂-聚酰胺联用富集纯化后,黄芪总黄酮提取量为310.63mg·g^-1,增加112倍,其中毛蕊异黄酮含量由0.13mg·g^-1增至42.56mg·g^-1,增加327倍;芒柄花素含量由0.06mg·g^-1增至7.89mg·g^-1,增加132倍。Flavonoids in astragali radix exert biological health functions actions such as antioxidant and immune-enhancing effects.They are often added to food and feed as immunopotentiators.The effects of ethanol concentration,the solid/liquid ratio,and extraction time on the extraction content of total flavonoids (TFA) from Astragalus membranaceus were evaluated using single factor experiments.Furthermore,the optimum beneficiation and depuration technology of TFA was investigated with HPD722 macroporous resin-polyamide combination method.The results showed that the extraction content of TFA and relative standard deviation (RSD) were 2.78 mg·g^-1 and 1.41%,respectively,under the conditions of 70% ethanol concentration,solid/liquid ratio of 1∶17,and an extraction time of 70 min.When TFA were enriched and purified using HPD722 macroporous resin-polyamide,the extraction content of TFA was 311 mg·g^-1 and its purity was increased 112 times.As the main component of TFA,the content of calycosin was increased 327 times from 0.13 mg·g^-1 to 42.56 mg·g^-1 and formononetin was increased 132 times from 0.06 mg·g^-1 to 7.89 mg·g^-1.

关 键 词：黄芪 黄酮 单因素试验 Box-Behnken法 大孔吸附树脂-聚酰胺联用 

分 类 号：S567.099[农业科学—中草药栽培]