糖尿病小鼠相关干眼眼表组织动态变化特征  

Dynamic changes of ocular surface tissue of diabetic dry eye in mice

作　　者：王茜[1] 万磊 李晶 周庆军 Wang Qian;Wan Lei;Li Jing;Zhou Qingjun(School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences,Jinan 250000,China;State Key Laboratory Cultivation Base,Shandong Provincial Key Laboratory of Ophthalmology,Shandong Eye Institute,Shandong First Medical University&Shandong Academy of Medical Sciences,Qingdao 266071,China)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,济南250000 [2]山东第一医科大学(山东省医学科学院)、山东省眼科研究所、山东省眼科学重点实验室一省部共建国家重点实验室培育基地,青岛266071

出　　处：《中华实验眼科杂志》2019年第6期419-424,共6页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金面上项目(81770904).

摘　　要：目的研究链脲佐菌素(STZ)腹腔内注射诱导的1型糖尿病小鼠干眼相关症状的发生和发展特征。方法SPF级6~8周龄雄性C57BL/6小鼠60只,按照随机数字表法随机分为对照组和糖尿病组,分别每日腹腔内注射枸橼酸盐缓冲液和50mg/kg STZ-枸橼酸盐缓冲液,连续注射5d。分别于注射前、末次注射后1、2、4个月检测各组小鼠体质量、血糖浓度、泪腺质量;同时采用酚红棉线法和虎红染色检测泪液分泌量和眼表情况;采用角膜知觉测量仪检测角膜敏感度;采用过碘酸希夫染色法检测结膜杯状细胞密度;采用组织病理学染色和Masson染色观察泪腺组织变化。结果糖尿病组小鼠注射后1、2和4个月体质量和泪腺质量与注射前比较,差异均无统计学意义(均P>0.05),但明显低于相应时间点对照组,差异均有统计学意义(均P<0.05)。糖尿病组小鼠注射后1、2和4个月血糖值明显高于注射前和相应时间点对照组,差异均有统计学意义(均P<0.05)。注射后1、2和4个月糖尿病组小鼠泪液分泌量明显低于注射前值和相应时间点对照组,差异均有统计学意义(均P<0.05)。注射后2个月糖尿病组小鼠眼表虎红染色开始出现阳性着色。注射后2个月和4个月糖尿病组小鼠角膜敏感度明显低于注射前和相应时间点对照组,差异均有统计学意义(均P<0.05)。注射后4个月糖尿病小鼠结膜杯状细胞密度明显低于注射前和相应时间点对照组,差异均有统计学意义(均P<0.05)。注射后4个月糖尿病组小鼠泪腺组织出现明显纤维化和炎性细胞浸润。结论STZ诱导的1型糖尿病小鼠早期主要表现为泪腺发育停滞,泪液分泌量下降,且随糖尿病病程延长而逐渐加重;后期出现角膜敏感度下降、眼表结构损伤、泪腺结构变化、杯状细胞密度下降等现象。Objective To explore the occurring and developing characteristics of dry eye syndrome in type 1 diabetic mouse model induced with streptozotocin (STZ)-intraperitoneal injection. Methods Completely randomized design method was performed.Sixty SPF degree male C57BL/6 mice (6-8 weeks old) was randomly divided into diabetic group and control group, which were intraperitoneally injected with citrate buffer and STZ-citrate buffer (50 mg/kg per day), respectively.The average weight, blood glucose level and lacrimal gland weight were examined before injection and 1 month, 2 months, 4 months after the last injection;meanwhile, phenol cotton thread and rose bengal staining methods were used to check tear formation and ocular surface condition;corneal perception meter was used to test corneal sensitivity;periodic acid-schiff (PAS) staining method was used to test the density of conjunctival goblet cells;histopathological staining and Masson staining methods were used to test the tissue changes of lacrimal gland. Results Compared with before injections, the body weight and lacrimal gland weight in diabetic group were not significantly changed 1 month, 2 months and 4 months after injection (all at P>0.05), but these measurements in diabetic group 1 month, 2 months and 4 months after injection were significantly lower than those in control group at corresponding time points (all at P<0.05). Compared with before injections and control group at corresponding time points, the blood glucose level were dramatically higher and the tear formation were significantly decreased in diabetic group at 1 month, 2 months, 4 months after injection (all at P<0.05). The ocular surface of diabetic model mice showed positive rose bengal staining 2 months after STZ injections.The corneal sensitivities were significantly lower in diabetic model mice 2 months and 4 months after injection than those before injection and in control group at corresponding time points (all at P<0.05). The density of conjunctival goblet cells in diabetic group 4 months a

关 键 词：糖尿病 干眼 泪液分泌 泪腺 角膜敏感度 

分 类 号：R587.2[医药卫生—内分泌] R777.34[医药卫生—内科学]