单因素考察甘草泻心汤超微饮片中甘草酸提取工艺研究  

Study on Extraction Technology of Glycyrrhizic Acid from Ultra-micro Pieces of Glycyrrhiza Xiexin Decoction by Single Factor Investigation

作　　者：吴柱[1] 谭军华 王灵芝[1] 魏丹丹[1,3] Wu Zhu;Tan Jun-hua;Wang Ling-zhi;Wei Dan-dan(Changsha Medical University, Hunan Changsha 410219;Hunan Jiudian Pharmaceutical Co.,Ltd., Hunan Changsha 410329;Hunan Yineng Biopharmaceutical Co.,Ltd., Hunan Changsha 410006)

机构地区：[1]长沙医学院,湖南长沙410219 [2]湖南省九典制药股份有限公司,湖南长沙410329 [3]湖南易能生物医药有限公司,湖南长沙410006

出　　处：《生物化工》2019年第3期53-55,59,共4页Biological Chemical Engineering

基　　金：2016年湖南省教育科学研究项目(湘教通[2016]395);2014年湖南省校企合作人才培养示范基地项目(湘教通[2014]272)

摘　　要：目的:优选甘草泻心汤超微饮片中甘草酸提取工艺,为其质量控制标准奠定基础。方法:以干膏得率为评价指标,考察甘草泻心汤超微饮片最佳提取工艺;采用HPLC法探究甘草泻心汤超微饮片甘草酸含量测定色谱条件。结果:以95%乙醇为溶剂采用连续回流提取法为较佳提取工艺;甘草酸的线性范围50~250μg/mL(R=0.9999),平均加样回收率为99.81%,RSD为1.95%(n=9)。结论:所建立方法操作简便、准确可靠。Objective To optimize the extraction technology of glycyrrhizic acid from ultra-micro pieces of Glycyrrhiza Xiexin decoction and lay a foundation for its quality control standard. Methods: Taking dry extract yielding rate as the evaluation index, the optimum extraction technology of ultra-micro pieces of Glycyrrhiza Xiexin decoction was investigated by single factor. The content of glycyrrhizic acid in was determined by HPLC. Results The optimum extraction process was 95 % ethanol as solvent, Continuous reflux as extraction method;2. In HPLC,the linear range for glycyrrhizic acid was 50 ~ 250 μg/mL(r=0.999), the average recovery was 99.81%,and its RSD was 1.95%(n=9). Conclusion The method is simple,accurate and reliable with good reproducibility, and can be used in the quality control of ultra-micro pieces of Glycyrrhiza Xiexin decoction.

关 键 词：甘草泻心汤 甘草酸 超微饮片 HPLC 

分 类 号：TQ461[化学工程—制药化工]