红松子油中皮诺敛酸的纯化及降脂活性评价  

Purification and Lipid-lowering Activity Evaluation of Pinoleic Acid in Korean Pine Seed Oil

作　　者：张晶 寇萍 秦启萍 于良 赵雪莲 王希清 杨雨春[2] 付玉杰 ZHANG Jing;KOU Ping;QING Qi-Ping;YU Liang;HAO Xue-Lian;WANG Xi-Qing;YANG Yu-Chun;FU Yu-Jie(Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040;Forestry Academy of Jilin Province,Changchun 130033)

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 [2]吉林省林业科学院,长春130033

出　　处：《植物研究》2019年第4期634-640,共7页Bulletin of Botanical Research

基　　金：“十三五”国家重点研发计划项目(2016YDF0600805)~~

摘　　要：以红松子油为原料,采用尿素包合法纯化皮诺敛酸,通过脂肪酸与尿素的比例、尿素与乙醇的比例、包合温度及包合时间等因素对皮诺敛酸含量及产率的影响,优化了尿素包合法的工艺参数,最终确定了皮诺敛酸的最佳纯化条件为:脂肪酸与尿素的比例为1∶6.5(w/w),尿素与90%乙醇的比例为1∶4(w/v),包合温度为-10℃,包合时间12h。在上述条件下,经GC-MS检测获得了纯度为58.3%的皮诺敛酸,回收率为51.8%,经二次尿素包合纯化后可获得纯度为72.5%的皮诺敛酸,回收率为64.7%。为了评估皮诺敛酸的降脂活性,以油酸诱导HepG2细胞成脂,最终确定40μmol·L^-1的皮诺敛酸能显著的降低HepG2细胞内甘油三酯及总胆固醇的水平。With Korean pine seed oil we enriched and purifiedpinoleic acid by urea inclusion.Through the influence of the ratio of fatty acid to urea,the ratio of urea to ethanol,we optimized the inclusion temperature and the inclusion time on the content of pinoleic acid and recovery of pinoleic acid,the process parameters of urea bagging. Finally,the optimal purification condition of pinoleic acid was determined as follows: the ratio of fatty acid to urea was 1∶6.5(w/w);the ratio of urea to 90% ethanol was 1∶4(w/v);Inclusion temperature was -10℃;Inclusion time was 12 h. Under the above conditions,a purity of 58.3% pinoleic acid could be obtained,and the recovery rate was 51.8%. After purification by secondary urea inclusion,a purity of 72.5% of pinoleic acid could be obtained,and the recovery rate was 64.7%. To verify the lipid-lowering activity of pinoleic acid,oleic acid was used to induce lipogenesis in HepG2 cells. The results showed 40 μmol·L ^-1 pinoleic acid could significantly reduce the level of triglycerides and total cholesterol in HepG2 cells.

关 键 词：红松子油 皮诺敛酸 尿素包合法 降脂活性 

分 类 号：S791.247[农业科学—林木遗传育种]