人卵丘细胞PROK1表达与IVF- ET后胚胎发育及妊娠结局的关系  

Correlation of cumulus cells PROK1 protein level with human embryo quality and pregnancy outcome in IVF-ET patients

作　　者：何敏[1] 李朋粉[1] 赵冬梅[1] 项云改[1] 张丹[1] 张瑜[1] 肖冰[1] 李茜[1] 谭丽[1] HE Min;LI Pengfen;ZHAO Dongmei;XIANG Yungai;ZHANG Dan;ZHANG Yu;XIAO Bing;LI Qian;TAN Li(Department of Reproductive Medicine,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China)

机构地区：[1]郑州大学第二附属医院生殖医学部

出　　处：《东南大学学报（医学版）》2019年第3期471-477,共7页Journal of Southeast University(Medical Science Edition)

基　　金：郑州市技术研究与开发经费支持项目(153pkjgg163)

摘　　要：目的:探讨人卵丘细胞前动力蛋白1(PROK1)表达与体外受精-胚胎移植(IVF-ET)后胚胎发育及妊娠结局的关系。方法:选择162例行IVF-ET的不孕症患者作为研究对象,蛋白质印迹法检测所有患者卵丘细胞PROK1表达,按照PROK1表达值分为PROK1高表达组和低表达组,比较两组患者一般资料、胚胎发育和妊娠结局,以及p53、活化型含半胱氨酸的天冬氨酸蛋白水解酶-3(caspase-3)、磷酸烯醇式丙酮酸羧激酶1(PCK1)蛋白表达和卵丘细胞葡萄糖含量的差异。结果:高表达组基础卵泡生成激素(FSH)值、卵母细胞成熟率、受精率、卵裂胚数、可利用胚胎数、优质胚胎数、种植率、临床妊娠率均明显高于低表达组(P<0.05),活化型caspase-3、PCK1蛋白及葡萄糖含量明显低于低表达组(P<0.05)。相关性分析显示卵丘细胞活化型caspase-3、PCK1蛋白及葡萄糖含量均与PROK1表达呈显著负相关(P<0.05)。结论:卵丘细胞PROK1表达与IVF-ET患者胚胎发育、临床妊娠结局有关,PROK1高表达有利于卵母细胞成熟和胚胎发育。Objective: To assess whether the levels of prokineticin 1(PROK) in cumulus cells derived from patients undergoing in vitro fertilization-embryo transfer(IVF-ET) were related to the embryo quality and clinical outcomes in IVF cycle. Methods: A total of one hundred and sixty-two IVF-ET patients were enrolled in this study, and the protein levels of PROK1 in cumulus cells were measured by Western blot. The patients were divided into two groups according to whether the PROK1 protein levels in their cumulus cells(CCs) were above or below the mean level detected in all the patients. The difference of clinical data,embryo quality and pregnancy outcome,as well as p53,cleaved-caspase-3 and PCK1 protein levels,between the two groups was compared. Moreover,relationship between PROK1 levels and glucose metabolism in CCs was also compared. Results: The basal follicle-stimulating hormone(FSH) level,retrieved oocyte,and the rate of maturiration,fertilization,cleavage,available embryo,high quality embryos,implantation and pregnancy were significantly higher in high PROK1 group than those in low PROK1 group,the differences between the two groups being statistically significant(all P <0.05). Moreover,the expression of cleaved-caspase-3,PCK1 protein and glucose content was also higher in high PROK1 group than that in low PROK1 group with statistically significant difference(all P <0.05). Correlation analysis demonstrated that cleaved-caspase-3,PCK1 protein levels and glucose contents were negatively correlated with PROK1 protein( P <0.05). Conclusion: The present findings imply that the PROK1 level in CCs is associated with embryo quality and pregnancy outcome of IVF-ET patients. Over-expression of PROK1 may have positive effects on oocyte maturation and embryo development.

关 键 词：卵丘细胞 前动力蛋白1 体外受精-胚胎移植 胚胎发育 妊娠结局 

分 类 号：R711.6[医药卫生—妇产科学]