松赤枯病病原的快速分子检测  

A Rapid Detection Method for the Pathogen of Pine Needle Blight

作　　者：袁川 吴南 刘应高[3] 许秀兰[3] 赵景阳[3] 李万艳[3] YUAN Chuan;WU Nan;LIU Ying-gao;XU Xiu-lan;ZHAO Jing-yang;LI Wan-yan(Forestry Technology Promotion Station,Guang'an,638000,China;Sichuan Association for Forest Integrated Pest Management,Chengdu,610081,China;College of Forestry,Sichuan Agricultural University,Chengdu,611130,China)

机构地区：[1]广安市林业技术推广站,四川广安638000 [2]四川省林业有害生物防治协会,四川成都610081 [3]四川农业大学林学院,四川成都611130

出　　处：《四川林业科技》2019年第3期77-81,共5页Journal of Sichuan Forestry Science and Technology

基　　金：四川省教育厅科研项目-四川几种重要林木真菌病害病原的分子检测技术研究(09ZA068)

摘　　要：为实现对松针中可导致松赤枯病的枯斑拟盘多毛孢的早期检测,依据枯斑拟盘多毛孢ITS区保守序列设计特异性引物AF(R/F),建立了松赤枯病PCR检测体系。结果表明该方法可于枯斑拟盘多毛孢基因组DNA与松针基因组DNA中扩增出大小为480 bp的单一条带,可从无明显症状的组织中检测到枯斑拟盘多毛孢。建立的PCR检测体系适用于枯斑拟盘多毛孢的分子鉴定及松赤枯病的早期诊断。In order to realize the early detection of Pestalotiopsis funerea in Pine needles,the specific primer AF(R/F) was designed,and PCR detection system was established according to the conserved sequence of ITS region of P.funerea.The results showed that the method could amplify a single band of 480 bp in genomic DNA of P.funerea and pine needle genomic DNA. P.funerea could be detected in tissues without obvious symptoms.The established PCR detection system was suitable for molecular identification of P.funerea and early diagnosis of pine needle blight.

关 键 词：枯斑拟盘多毛孢 松针基因组DNA 快速 分子检测 

分 类 号：S763.1[农业科学—森林保护学]