烤烟成熟期烟叶木质素合成关键酶基因的转录分析  

Transcriptional analysis of key enzyme genes in synthesis of lignin in flue-cured tobacco leaves during maturity stage

作　　者：高娅北 孙曙光[2] 王胜雷[3] 陈二龙 杨晓亮[2] 孙占伟 宋朝鹏[1] GAO Yabei;SUN Shuguang;WANG Shenglei;CHEN Erlong;YANG Xiaoliang;SUN Zhanwei;SONG Zhaopeng(College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China;China Tobacco Hubei Industrial Co. Ltd., Wuhan 430000, China;Fujian Provincial Tobacco Company, Fuzhou 350000, China;China Tobacco Henan Industrial Co. Ltd., Zhengzhou 450016, China)

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]湖北中烟工业有限责任公司,湖北武汉430040 [3]福建省烟草公司,福建福州350003 [4]河南中烟工业有限责任公司,河南郑州450016

出　　处：《中国烟草学报》2019年第3期43-51,共9页Acta Tabacaria Sinica

基　　金：湖北中烟工业有限责任公司资助项目“黄鹤楼原料需求区域拓展技术研究及应用”(2018A011YL05);河南中烟工业有限责任公司科技创新项目“碳氮专用复合肥工程研发”(ZW2014005)

摘　　要：木质素对烟叶抗性形成具有重要意义,但含量过高会降低卷烟产品品质。为系统探究木质素在烟叶成熟期合成积累的关键点及调控基因的变化规律,本研究以 K326 为试验材料,测定烤烟中部叶成熟期叶片硬度、组织结构、木质素含量及相关合成酶基因的表达模式。结果表明,成熟期是烤烟木质素合成积累的重要时期,在整个成熟期后烟叶木质素含量和硬度呈现先增加后降低的变化规律,在打顶后 30 天,木质素含量和烟叶硬度达到最大值;烟叶成熟期木质素合成酶相关基因的 qRT-PCR 表达分析表明:PAL、C4H、4CL1、CCR1、CCoAOMT5、COMT1、CAD2、PAO1 和 F5H1 基因表达规律与木质素变化规律较为一致,呈现先增加后降低的变化趋势,且在打顶后 30 天,表达量达到峰值。其中 PAL、C4H、CCR1、CAD2 和 PAO1 基因对烟叶木质素合成积累的贡献较大(p<0.01,r=0.93, 0.90, 0.91, 0.90, 0.89;P=0.24, 0.19, 0.16, 0.17, 0.19),是烟叶木质素合成积累过程中的关键调控基因。Lignin plays an important role in the formation of tobacco leaf resistance, but excessive content of lignin affect quality of cigarette products. In order to systematically explore the key points for lignin synthesis and accumulation in tobacco leaves at maturity stage as well as the change of regulatory genes, this study analyzes leaf hardness, tissue structure, lignin content and expression patterns of lignin synthase-related genes in middle leaves of flue-cured tobacco at maturity stage using K326 as experimental material. Results showed that maturity stage is an important period for the accumulation of lignin in flue-cured tobacco;the lignin content and hardness of tobacco leaves increased first and then decreased after entering maturity period and then reached the maximum 30 days after topping. Results of quantitative RT-PCR analysis of lignin synthase-related genes in mature tobacco leaves indicated that the expression patterns of PAL, C4H, 4CL1, CCR1, CCoAOMT5, COMT1, CAD, PAO1 and F5H1 were consistent with that of lignin changes, i.e. showing a trend of increasing first and then decreasing, and reaching its peak 30 days after topping. Among them, PAL, C4H, CCR1, CAD2 and PAO1 genes contributed more to lignin synthesis and accumulation in tobacco leaves (p<0.01, r=0.93, 0.90, 0.91, 0.90, 0.89;P=0.24, 0.19, 0.16, 0.17, 0.19), which can be regarded as key regulatory genes for lignin synthesis and accumulation in middle leaves of flue-cured tobacco.

关 键 词：烤烟 木质素 基因表达模式 成熟期 

分 类 号：S572[农业科学—烟草工业]