末端回肠组蛋白脱乙酰基酶3表达与肥胖以及肠道菌群关系的初步探索  

Preliminary Study on Relationship Between Terminal Ileal Histone Deacetylase 3 Expression and Obesity and Intestinal Flora

作　　者：范俊杰[1] 曾悦[1] 黄春兰[1] 陆颖影[1] FAN Junjie;ZENG Yue;HUANG Chunlan;LU Yingying(Department of Gastroenterology,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai,201620)

机构地区：[1]上海交通大学附属第一人民医院消化内科

出　　处：《胃肠病学》2019年第5期264-268,共5页Chinese Journal of Gastroenterology

基　　金：国家自然科学基金青年科学基金项目(81600500)

摘　　要：背景:表达于肝脏、肌肉和脂肪组织中的组蛋白脱乙酰基酶3(HDAC3)与脂肪代谢密切相关;而肠上皮细胞中的HDAC3可调节宿主与肠道菌群之间的相互作用,维持肠道内环境稳态。目的:探讨末端回肠HDAC3表达与肥胖以及肠道菌群的关系。方法:8只6周龄SPF C57BL/6小鼠和8只6周龄无菌C57BL/6小鼠分别随机分为普通饮食组和高脂饮食组,予喂饲相应饲料5周,每周记录体质量变化。获取末端回肠组织,以免疫组化染色和realtime PCR检测HDAC3蛋白和mRNA表达。结果:与SPF普通饮食组相比,SPF高脂饮食组小鼠体质量增长明显(P<0.05),末端回肠HDAC3蛋白和mRNA表达均显著增高(P<0.05)。无菌高脂饮食组和无菌普通饮食组小鼠实验过程中体质量无明显变化(P>0.05),两组间HDAC3蛋白和mRNA表达差异无统计学意义(P>0.05)。结论:肠道菌群对末端回肠HDAC3表达可能具有潜在调节作用,进而参与肥胖发生。Background:Histone deacetylase 3(HDAC3),which is expressed in liver,muscle and adipose tissue,is closely related to fat metabolism.Furthermore,HDAC3 expressed in intestinal epithelial cells is a critical factor that regulates hostcommensal flora relationship and maintains intestinal homeostasis.Aims:To investigate the relationship between HDAC3 expression in terminal ileum and obesity and intestinal flora.Methods:Eight SPF C57 BL/6 mice and eight germ-free C57 BL/6 mice aged six weeks were randomly placed on a standard chow or a high-fat chow,respectively,for 5 weeks.Changes in body weight were recorded weekly,and the terminal ileum was obtained for detection of HDAC3 protein and mRNA expressions by immunohistochemistry and real-time PCR.Results:Increase of body weight of mice in SPF high-fat diet group was much more than that in SPF normal diet group(P<0.05).Meanwhile,HDAC3 protein and mRNA expressions in terminal ileum in SPF high-fat diet group was significantly higher than those in SPF normal diet group(P<0.05).On the other hand,whether fed with high-fat diet or normal diet,no significant change of body weight occurred in germ-free mice during the 5-week feeding(P>0.05).No significant differences were found in HDAC3 protein and mRNA expressions between germ-free high-fat diet group and germ-free normal diet group(P>0.05).Conclusions:Expression of HDAC3 in terminal ileum might be regulated by intestinal flora and participates in the occurrence of obesity.

关 键 词：组蛋白脱乙酰基酶3 膳食 高脂 肥胖症 肠道菌群 无菌生物 小鼠 近交C57BL 

分 类 号：R589.2[医药卫生—内分泌]