貂源停乳链球菌似马亚种的分离鉴定  

Isolation and Identification of Streptococcus dysgalactiae subsp. equisimilis from Mink

作　　者：陈欣婷 李清欣 张彦龙 CHEN Xin-ting;LI Qing-xin;ZHANG Yan-long(College of Wildlife Resource,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学野生动物资源学院

出　　处：《畜牧与饲料科学》2019年第6期99-103,共5页Animal Husbandry and Feed Science

基　　金：国家林业局野生动植物保护管理项目(野生动物疫源疫病病原体分析与保持)

摘　　要：为了研究引起水貂肺炎死亡的病因,对送检的病死水貂进行剖检。从病死水貂病料中分离出了一株革兰阳性球菌,对其进行菌落形态观察、生化鉴定以及16SrDNA序列分析,构建系统进化树并进行同源性分析,同时对该菌株进行药敏试验。结果表明:根据分离菌的形态特征、生物化学特性,结合16SrDNA序列测定与进化树分析,将其鉴定为链球菌属的停乳链球菌似马亚种。药敏试验结果显示,该菌株对青霉素、阿莫西林、莫西沙星等药物敏感,但对四环素有较强的耐药性。In order to determine the etiological agent associated with the death of mink with pneumonia, the dead mink was autopsied. A gram-positive cocci was isolated from the clinical sample, and it was subsequently subjected to colony morphology observation, biochemical identification and 16S rDNA sequence analysis. The phylogenetic tree based on the 16S rDNA sequence was constructed, and the homology analysis was performed. The antimicrobial sensitivity test of the isolated strain was also carried out. The results showed that based on the morphological and biochemical characteristics of the isolate, combined with 16S rDNA sequencing and phylogenetic tree analysis, the isolates were identified as Streptococcus dysgalactiae subsp. equisimilis. Antimicrobial sensitivity test showed that the strain was sensitive to penicillin, amoxicillin, moxifloxacin and some other antibiotics, but had strong resistance to tetracycline.

关 键 词：停乳链球菌似马亚种 水貂 分离 鉴定 

分 类 号：S855.11[农业科学—临床兽医学]