液相色谱柱串联技术高效分离酒样中的手性芳香氨基酸  

Effective Separation of Aromatic Amino Acids in Alcohol Samples by C18 Column Tandem Crown Ether Chiral Column

作　　者：许志刚[1] 王丽 刘育坚 刘宏程[2] 司晓喜 XU Zhigang;WANG Li;LIU Yujian;LIU Hongcheng;SI Xiaoxi(Faculty of Science,Kunming University of Science and Technology,Kunming 650500,China;Institute of Quality Standard and Testing Technology,Yunnan Academy of Agriculture Science,Kunming 650223,China;R&D Center of China Tobacco Yunnan Industrial Co. Ltd.,Kunming 650231,China)

机构地区：[1]昆明理工大学理学院,云南昆明650500 [2]云南省农业科学院质量标准与检测技术研究所,云南昆明650223 [3]云南中烟工业有限责任公司技术中心,云南昆明650231

出　　处：《昆明理工大学学报（自然科学版）》2019年第3期102-107,共6页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：国家自然科学基金项目(21565018);云南省重点研发计划项目(2018BC005);昆明理工大学校级学科方向团队项目(14078356);昆明理工大学大学生课外学术科技创新基金(2018BA387)

摘　　要：建立C18柱串联CR-I(+)手性冠醚柱的反相高效液相色谱分离方法,用于同时拆分三种芳香氨基酸对映体.研究了流动相比例、流速、柱温以及色谱柱的串联次序对三种芳香氨基酸对映体拆分的影响.实验结果表明:当流动相为pH2.0的高氯酸溶液-乙腈(85∶15,体积比),流速为0.4mL/min,柱温为21℃,串联顺序为手性冠醚柱在前、C18柱在后时三种芳香氨基酸对映体分离效果最优.在最优色谱条件下,分离并测定了米酒和啤酒样品中的三类芳香氨基酸对映体.A reversed phase chromatographic method of C18 column tandem chiral crown ether column was developed to simultaneously separate three aromatic amino acids enantiomers. The conditions, including mobile phase, flow rate, column temperature and column tandem order, were optimized. The best separation results could be obtained under the conditions of perchloric acid solution(pH 2.0)-acetonitrile(85∶15, volume ratio) mobile phase, 0.4 mL/min flow rate, 21 ℃ column temperature, and the column order of chiral crown ether column tandem C18 column. Three aromatic amino acids in rice wine and beer samples were separated and determined by the proposed method.

关 键 词：柱串联 芳香氨基酸 手性分离 酒类样品 

分 类 号：O657.7[理学—分析化学]